| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
We have developed a novel culture method for human colonic epithelial cells that allows long-term maintenance and efficient expansion of stem cells in vitro from tiny biopsy specimens. The factors used in this method were successfully replaced to GMP (Good Manufacturing Practice)-grade recombinant proteins designed to use for clinical regenerative medicine. We also refined the transplantation method for colonic epithelial cells onto colon and developed a new method for epithelial cell transplantation. With this method, we found that cultured small intestinal cells could be heterotopically engrafted onto the colon, which means that the method would be a good model for cultured human colonic cell transplantation.
These results would be of significant help to build a basis for regenerative medicine using cultured human colonic epithelial stem cells.
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