Development of serum Helicobacter pylori CagA antibody detection kit
Project/Area Number |
25860544
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Gastroenterology
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Research Institution | Oita University |
Principal Investigator |
SHIOTA SEIJI 大分大学, 医学部, 医員 (00420646)
|
Project Period (FY) |
2013-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
Keywords | ヘリコバクター・ピロリ / CagA / ピロリ / ピロリ菌 |
Outline of Final Research Achievements |
We previously reported that Helicobacter pylori CagA was significantly associated with gastric cancer in East Asian countries. In addition, we reported that serum CagA antibody titers were correlated with the severity of gastritis. In this study, we purified H. pylori full length CagA protein using E. coli and developed a rapid detection kit for serum CagA antibody based on immunochromatography. The preliminary test kits showed positive bands for H. pylori-positive/CagA Ab positive cases, and showed negative for H. pylori-negative/CagA Ab negative cases. Appropriate condition for serum samples for H. pylori-positive but CagA Ab negative is now in progress.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Differences in interleukin 8 expression in Helicobacter pylori-infected gastric mucosa tissues from patients in Bhutan and the Dominican Republic2015
Author(s)
Nagashima H, Iwatani S, Cruz M, Jiménez Abreu JA, Tronilo L, Rodríguez E, Disla M, Terao H, Uchida T, Mahachai V, Vilaichone RK, Tshering L, Mitsui T, Shiota S, Graham DY, Yamaoka Y
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Journal Title
Hum Pathol
Volume: 46
Issue: 1
Pages: 129-136
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Virulence genes of Helicobacter pylori in the Dominican Republic2014
Author(s)
Shiota S, Cruz M, Abreu JA, Mitsui T, Terao H, Disla M, Iwatani S, Nagashima H, Matsuda M, Uchida T, Tronilo L, Rodríguez E, Yamaoka Y
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Journal Title
J Med Microbiol
Volume: 63
Issue: 9
Pages: 1189-1196
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Identification of Helicobacter pylori infection in symptomatic patients in Surabaya, Indonesia, using five diagnostic tests2014
Author(s)
Miftahussurur M, Shiota S, Suzuki R, Matsuda M, Uchida T, Kido Y, Kawamoto F, Maimunah U, Adi P, Rezkitha Y, Nasronudin, Nusi I, Yamaoka Y
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Journal Title
Epidemiol Infect.
Volume: 143
Issue: 5
Pages: 986-996
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Histological characteristics of gastric mucosa prior to Helicobacter pylori eradication may predict gastric cancer.2013
Author(s)
Kodama M, Murakami K, Okimoto T, Abe H, Sato R, Ogawa R, Mizukami K, Shiota S, Nakagawa Y, Soma W, Arita T, Fujioka T.
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Journal Title
Scand J Gastroenterol
Volume: 48
Issue: 11
Pages: 1249-1256
DOI
Related Report
Peer Reviewed