Effects of Helicobacter pylori virulence genotypes on disease outcomes

• Project/Area Number: 25860545
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Oita University
• Principal Investigator: MATSUDA Miyuki 大分大学, 医学部, 技術補佐員 (90586207)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: ヘリコバクター・ピロリ / 病原因子 / 遺伝子変異株 / ピロリ菌

Outline of Final Research Achievements

It is important to identify the high risk group of peptic ulcers and gastric cancer infected with Helicobacter pylori. The most studied virulence factors of H. pylori to cause these diseases is cagA. In this project, we performed PCR-based sequencing of cagA from cultured clinical isolates of H. pylori. We could detect various genotypes of cagA including AB, ABC, ABCC, ABCCC, ABD, ABABCC. We co-cultured these types of wild-type H. pylori or cagA-deleted mutants with gastric epithelial cells, and performed microarray to identify the molecules up-regulated or down-regulated, and the analyses are now on going.

Research Products

• [Journal Article] Prevalence of Helicobacter pylori infection and atrophic gastritis in patients with dyspeptic symptoms in Myanmar (2015)
  • Author(s): Myint T, Shiota S, Vilaichone RK, Ni N, Aye TT, Matsuda M, Tran TT, Uchida T, Mahachai V, Yamaoka Y.
  • Journal Title: World J Gastroenterol Volume: 21 Issue: 2 Pages: 629-636
  • DOI: 10.3748/wjg.v21.i2.629
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Extremely low Helicobacter pylori prevalence in North Sulawesi, Indonesia and identification of a Maori-tribe type strain: a cross sectional study (2014)
  • Author(s): Miftahussurur M, Tuda J, Suzuki R, Kido Y, Kawamoto F, Matsuda M, Tantular IS, Pusarawati S, Nasronudin, Harijanto PN, Yamaoka Y
  • Journal Title: Gut Pathog Volume: 6 Issue: 1
  • DOI: 10.1186/s13099-014-0042-0
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Identification of Helicobacter pylori infection in symptomatic patients in Surabaya, Indonesia, using five diagnostic tests (2014)
  • Author(s): Miftahussurur M, Shiota S, Suzuki R, Matsuda M, Uchida T, Kido Y, Kawamoto F, Maimunah U, Adi P, Rezkitha Y, Nasronudin, Nusi I, Yamaoka Y
  • Journal Title: Epidemiol Infect. Volume: 143 Issue: 5 Pages: 986-996
  • DOI: 10.1017/s095026881400154x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Virulence genes of Helicobacter pylori in the Dominican Republic (2014)
  • Author(s): Shiota S, Cruz M, Abreu JA, Mitsui T, Terao H, Disla M, Iwatani S, Nagashima H, Matsuda M, Uchida T, Tronilo L, Rodríguez E, Yamaoka Y
  • Journal Title: J Med Microbiol Volume: 63 Issue: 9 Pages: 1189-1196
  • DOI: 10.1099/jmm.0.075275-0
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequences of Eight Helicobacter pylori Strains with Different Virulence Factor Genotypes and Methylation Profiles, Isolated from Patients with Diverse Gastrointestinal Diseases on Okinawa Island, Japan, Determined Using PacBio Single-Molecule Real-Time Technology (2014)
  • Author(s): Satou K, Shiroma A, Teruya K, Shimoji M, Nakano K, Juan A, Tamotsu H, Terabayashi Y, Aoyama M, Teruya M, Suzuki R, Matsuda M, Sekine A, Kinjo N, Kinjo F, Yamaoka Y, Hirano T
  • Journal Title: Genome Announc Volume: 2 Issue: 2
  • DOI: 10.1128/genomea.00286-14
  • Peer Reviewed / Open Access
• [Journal Article] Discovery of novel mutations for clarithromycin resistance in Helicobacter pylori by using next-generation sequencing (2014)
  • Author(s): Binh TT, Shiota S, Suzuki R, Matsuda M, Trang TT, Kwon DH, Iwatani S, Yamaoka Y
  • Journal Title: J Antimicrob Chemother Volume: 69 Issue: 7 Pages: 1796-1803
  • DOI: 10.1093/jac/dku050
  • Peer Reviewed / Acknowledgement Compliant