Comprehensive analysis of the origin of colorectal cancer based on intratumoral genetic heterogeneity

• Project/Area Number: 25860547
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Sapporo Medical University
• Principal Investigator: YAMAMOTO Eiichiro 札幌医科大学, 医学部, 助教 (60567915)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 大腸がん / 遺伝子変異 / メチル化 / 大腸癌

Outline of Final Research Achievements

We analyzed the intratumoral variability of the endoscopic and genetic alterations in colorectal tumors. Our exome sequencing identified a large number of somatic mutations and revealed that most somatic mutations were not commonly observed among the two or more subcomponents with different endoscopic features.

Research Products

• [Journal Article] Analysis of DNA Methylation in Bowel Lavage Fluid for Detection of Colorectal Cancer. (2014)
  • Author(s): Harada T, Yamamoto E, Yamano HO, Nojima M, Maruyama R, Kumegawa K, Ashida M, Yoshikawa K, Kimura T, Harada E, Takagi R, Tanaka Y, Aoki H, Nishizono M, Nakaoka M, Tsuyada A, Niinuma T, Kai M, Shimoda K, Shinomura Y, Sugai T, Imai K, Suzuki H.
  • Journal Title: Cancer Prev Res (Phila) Volume: 7 Pages: 1002-10
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators. (2014)
  • Author(s): Tahara T, Yamamoto E, Madireddi P, Suzuki H, Maruyama R, Chung W, Garriga J, Jelinek J, Yamano HO, Sugai T, Kondo Y, Toyota M, Issa JP, Estécio MR.
  • Journal Title: Gastroenterology Volume: 146 Issue: 2 Pages: 530-8
  • DOI: 10.1053/j.gastro.2013.10.060
  • Peer Reviewed
• [Journal Article] Colorectal carcinomas with CpG island methylator phenotype 1 frequently contain mutations in chromatin regulators (2014)
  • Author(s): Tahara T, Yamamoto E, Madireddi P, Suzuki H, Maruyama R, Chung W, Garriga J, Jelinek J, Yamano HO, Sugai T, Kondo Y, Toyota M, Issa JP, Estecio MR
  • Journal Title: Gastroenterology Volume: 146 Issue: 5 Pages: 530-538
  • DOI: 10.1158/0008-5472.can-13-1865
  • Peer Reviewed
• [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk (2013)
  • Author(s): Suzuki R, Nosho K (他14名), Yamamoto H, Shinomura Y
  • Journal Title: J Gastroenterol Volume: (Epub ahead of print) Issue: 7 Pages: 1135-1144
  • DOI: 10.1007/s00535-013-0861-7
  • Peer Reviewed / Open Access
• [Journal Article] Aberrant methylation of microRNA-34b/c is a predictive marker of metachronous gastric cancer risk. (2013)
  • Author(s): Suzuki R1, Yamamoto E, Nojima M, Maruyama R, Yamano HO, Yoshikawa K, Kimura T, Harada T, Ashida M, Niinuma T, Sato A, Nosho K, Yamamoto H, Kai M, Sugai T, Imai K, Suzuki H, Shinomura Y.
  • Journal Title: J Gastroenterol. Volume: Epub ahead of print Pages: 9999-9999
  • Peer Reviewed
• [Presentation] ANALYSIS OF DNA METHYLATION IN BOWEL LAVAGE FLUID FOR DETECTION OF COLORECTAL CANCER (2014)
  • Author(s): Eiichiro Yamamoto
  • Organizer: UEG Week 2014
  • Place of Presentation: ウィーン（オーストリア）
  • Year and Date: 2014-10-18 – 2014-10-20
• [Presentation] Histone H3K27 trimethylation in normal colon is associated with developing colorectal tumor with CpG island methylator phenotype (2014)
  • Author(s): Eiichiro Yamamoto
  • Organizer: 日本癌学会
  • Place of Presentation: パシフィコ横浜（横浜）
  • Year and Date: 2014-09-26 – 2014-09-27
• [Presentation] Intratumoral variation in surface microstructures reflects genetic heterogeneity revealed by exome sequencing of colorectal tumor.
  • Author(s): Eiichiro Yamamoto
  • Organizer: 日本癌学会
  • Place of Presentation: 横浜
• [Presentation] INTRATUMORAL VARIATIONS IN PIT PATTERNS REFLECT GENETIC HETEROGENEITY REVEALED BY HIGH- THROUGHPUT SEQUENCING OF COLORECTAL TUMOR
  • Author(s): Eiichiro Yamamoto
  • Organizer: UEGW
  • Place of Presentation: ベルリン