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Analysis of a novel mitochondrial protein AMF in energy metabolism

Research Project

Project/Area Number 25860599
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cardiovascular medicine
Research InstitutionOsaka University

Principal Investigator

KATO Hisakazu  大阪大学, 医学(系)研究科(研究院), 特任研究員 (30589312)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsミトコンドリア / ATP / 虚血 / 循環器・高血圧 / 分子心臓学 / エネルギー代謝
Outline of Final Research Achievements

Heart tissue consumes more energy than other organs to maintain cardiac pump function. Imbalances between energy demand and supply in an ischemic myocardium fall into the mechanical failure of heart. However, the mechanism by which mitochondrial ATP production is regulated under hypoxia is not fully understood. In this study, we revealed how a novel hypoxia-induced protein AMF affects mitochondrial ATP production under ischemic condition, mainly from the following experiments. (1) Immunoaffinity purification and mass spectrometric analysis revealed that AMF interacted with FoF1-ATP synthase complex. (2) Assessment of mitochondrial ATP concentration using FRET-based ATP biosensor allowed us to show that overexpression of AMF inhibited the decreases in mitochondrial ATP concentration under hypoxic condition in cultured cardiomyocytes and also in zebrafish heart. These results suggest that AMF functions as a guardian of ischemic heart via activating FoF1-ATP synthase.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (8 results)

All 2015 2014 2013 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (2 results) Remarks (2 results)

  • [Journal Article] Augmented AMPK activity inhibits cell migration by phosphorylating the novel substrate Pdlim5.2015

    • Author(s)
      Yan Y, Tsukamoto O, Nakano A, Kato H, Kioka H, Ito N, Higo S, Yamazaki S, Shintani Y, Matsuoka K, Liao Y, Asanuma H, Asakura M, Takafuji K, Minamino T, Asano Y, Kitakaze M, Takashima S.
    • Journal Title

      Nat Commun.

      Volume: 6 Issue: 1 Pages: 6137-6137

    • DOI

      10.1038/ncomms7137

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Higdla is a oositive regulaator of cytochrome c oxidase2015

    • Author(s)
      T. Hayashi, Y. Asano, Y. Shintani, H. Aoyama, H. Kioka, O. Tsukamoto, M. Hikita, K. Shinzawa-Itoh, K. Takajuji, S. Higo, H. Kato, S. Yamazaki, K. Matsuoka, A. Nakano, H. Asanuma, M. Asakura, T. Minamino, Y. Goto, T. Ogura, M. Kitakaze, I. Komuro, Y. Sakata, T. Tsukihara, S. Yoshikawa, S. Takashima
    • Journal Title

      Proc. Nat. Acad. Sci. U.S.A.

      Volume: 112 Issue: 5 Pages: 1553-1558

    • DOI

      10.1073/pnas.1419767112

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Noninvasive and quantitative live imaging reveals a potential stress-responsive enhancer in the failing heart.2014

    • Author(s)
      Matsuoka K, Asano Y, Higo S, Tsukamoto O, Yan Y, Yamazaki S, Matsuzaki T, Kioka H, Kato H, Uno Y, Asakura M, Asanuma H, Minamino T, Aburatani H, Kitakaze M, Komuro I, Takashima S.
    • Journal Title

      FASEB J.

      Volume: 28(4) Issue: 4 Pages: 1870-9

    • DOI

      10.1096/fj.13-245522

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Evaluation of intra-mitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation2013

    • Author(s)
      Kioka H, Kato H, Fujikawa M, Tsukamoto O, Suzuki T, Imamura H, Nakano A, Higo S, Yamazaki S, Matsuzaki T, Tkafuji K, Asanuma H, Asakura M, Minamino T, Shintani Y, Yoshida M, Noji H, Kitakaze M, Komuro I, Asano Y and Takashima S
    • Journal Title

      PNAS

      Volume: 111 Issue: 1 Pages: 273-278

    • DOI

      10.1073/pnas.1318547111

    • Related Report
      2013 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Evaluation of Mitochondrial ATP Levels in Vivo Identifies G0/G1 Switch Gene 2 as a Therapeutic Target of Ischemic Heart Failure2014

    • Author(s)
      Hisakazu KATO, Hidetaka KIOKA, Yoshihiro ASANO, Seiji TAKASHIMA
    • Organizer
      第18回日本心不全学会学術集会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2014-10-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] hypoxia-inducible protein MENT enhances mitochondrial ATP production by interacting with FoF1-ATP synthase2013

    • Author(s)
      Hisakazu KATO, Hidetaka Kioka, Makoto Fujikawa, Toshiharu Suzuki, Hiromi Imamura, Masasuke Yoshida, Yoshihiro Asano, Seiji Takashima
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸 ポートアイランド
    • Related Report
      2013 Research-status Report
  • [Remarks] 大阪大学大学院医学系研究科・生命機能研究科 医化学講座

    • URL

      http://medbio.sakura.ne.jp/

    • Related Report
      2014 Annual Research Report
  • [Remarks] 大阪大学大学院医学系研究科 医化学講座

    • URL

      http://www.medbio.med.osaka-u.ac.jp/

    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

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