| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
We analyzed the role of an androgen receptor (AR) signal for the progression of kidney diseases using mouse models. Immunostaining revealed that the androgen receptors were localized in the nucleus of proximal tubular cells. Next, we found that exogenous administration of testosterone to hemi-nephrectomised wild type (WT) mice induced albumiuria and high blood pressure. On the other hand, expression levels of α-smooth muscle actin and COL1A1 mRNA in unilateral ureteral obstruction kidneys of AR-knockout (ARKO) mice were higher than those of WT mice. Further, when the ARKO and WT mice were subjected to hemi-nephrectomies and ischemia-reperfusions of the contralateral kidneys, the serum levels of urea nitrogen at 24 hours after re-perfusion were higher in ARKO mice than those in WT mice. Our results suggest that both excess and deficiency of the AR signal are associated with progression of kidney diseases.
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