| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we generated double reporter hiPSC lines, allowing us to monitor and isolate OSR1+SIX2+ nephron progenitors. We then established a multistep differentiation protocol from hiPSCs into OSR1+SIX2+ nephron progenitors with the induction efficiency nearly 40%. These human nephron progenitors differentiate into multiple renal epithelia including glomerular podocytes and tubular cells, and reconstitute three-dimensional renal structures in vitro and in vivo. Moreover, we found that renal subcapsular transplantation of hiPSC-derived OSR1+SIX2+ nephron progenitors ameliorated acute kidney injury in mice induced by ischemic reperfusion injury. Our results suggest that regenerative medicine strategies for kidney diseases could be developed using the hiPSC-derived renal cells.
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