The development of differentiation method that human induced pluripotency stem cells differentiate into mature renal cells as clinical applications.

Research Project

Project/Area Number	25860675
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Kidney internal medicine
Research Institution	Okayama University (2014) Kyoto University (2013)
Principal Investigator	INOUE Tatsuyuki 岡山大学, 医歯(薬)学総合研究科, 助教 (60598564)
Project Period (FY)	2013-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	iPS細胞 / 腎前駆細胞
Outline of Final Research Achievements	We aimed to develop a method for differentiating hiPSCs (induced pluripotency stem cells) into mature renal cells. It became necessary to prepare a renal progenitor cells in large scale and stable, we had to change the aim to the development of efficient method that iPSCs differentiated into renal progenitor cells. We performed high-throughput screening of chemical compounds and identified several compounds. In second screening, we identified 3 compounds as hit compounds. These compounds had a dose-dependency, and affected the mouse embryonic kidney. We are going to analyze the function of these compounds in developing kidney, and also establish the highly efficient differentiation method.

Report

(3 results)