| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
To analyze the function of fibroblasts and proximal tubular epithelial cells in the kidney, we utilized Cre recombination and diphtheria toxin receptor (DTR) system. DT administration for the mice expressing DTR in fibroblasts triggered the expression of tubular injury markers, as well as the proliferation of proximal tubule cells. To search for the molecules supporting tubular cells, we performed microarray analysis and identified several signaling pathways down-regulated by DT administration.
Meanwhile, DT administration for the mice DTR expressing in proximal tubular cells caused severe proximal tubule-specific injury associated with interstitial fibrosis. Mild proximal tubule injury from a single injection of low-dose DT triggered reversible fibrosis, whereas repeated mild injuries caused sustained interstitial fibrosis.
These results support the existence of crosstalk between fibroblasts and tubular cells, and the signaling pathways may be the target for new therapeutics.
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