• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Pathological significance of Nox isoforms of NADPH oxidase in diabetic nephropathy

Research Project

Project/Area Number 25860694
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Kidney internal medicine
Research InstitutionKawasaki Medical School

Principal Investigator

NAMIKOSHI Tamehachi  川崎医科大学, 医学部, 講師 (90509332)

Co-Investigator(Renkei-kenkyūsha) COOPER Mark  Baker IDI Heart and Diabetes Institute, Prof.
CHANNON Keith  Oxford University, Prof.
TANNER George  Indiana University, Prof.
Research Collaborator KASHIHARA Naoki  川崎医科大学, 医学部, 教授 (10233701)
SATOH Minoru  川崎医科大学, 医学部, 准教授 (70449891)
KUWABARA Atsunori  川崎医科大学, 医学部, 講師 (50368627)
YORIMITSU Daisuke  川崎医科大学, 医学部, 臨床助教 (50412177)
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords糖尿病性腎症 / NADPHオキシダーゼ / Nox / オーストラリア:アメリカ
Outline of Final Research Achievements

Activated NADPH oxidase is associated with progression of diabetic nephropathy, but the pathological significance of Nox isoforms, cell membrane components of NADPH oxidase, remains to be elucidated. Here, we investigated ameliorative effects of genetic manipulation of Nox1 and Nox4, on diabetic mice kidneys, to clarify the role of Nox isoforms in diabetic nephropathy. Diabetic/control knockout (KO)/wild-type mice were used for the examination at ~20 weeks after induction of diabetes. Diabetes-induced albuminuria was attenuated in both Nox1KO and Nox4KO mice, while the glomerular sclerosis was mitigated in only Nox1KO kidneys. Increased mRNA expressions of Nox2, fibronectin, and alpha-SMA in diabetic kidneys were suppressed in only Nox1KO mice. Accumulation of nitrotyrosine in glomeruli of diabetic kidneys was completely suppressed in Nox1KO mice, although it was reduced insufficiently in Nox4KO mice. Collectively, activated Nox1 may contribute to progression of diabetic nephropathy.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (2 results)

All 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results)

  • [Journal Article] Genetic targeting or pharmacologic inhibition of NADPH oxidase Nox4 provides renoprotection in long-term diabetic nephropathy2014

    • Author(s)
      Jay C. Jha, Stephen P. Gray, David Barit, Jun Okabe, Assam El-Osta, Tamehachi Namikoshi, Vicki Thallas-Bonke, Kirstin Wingler, Cedric Szyndralewiez, Freddy Heitz, Rhian M. Touyz, Mark E. Cooper, Harald H.H.W. Schmidt, Karin A. Jandeleit-Dahm
    • Journal Title

      JOURNAL OF AMERICAN SOCIETY OF NEPHROLOGY

      Volume: 25 Issue: 6 Pages: 1237-1254

    • DOI

      10.1681/asn.2013070810

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of Nox2 in diabetic kidney disease2013

    • Author(s)
      Young-Hyun You, Shinichi Okada, San Ly, Karin Jandeleit-Dahm, David Barit, Tamehachi Namikoshi, Kumar Sharma
    • Journal Title

      AMERICAN JOURNAL OF PHYSIOLOGY RENAL PHYSIOLOGY

      Volume: 304巻 Issue: 7 Pages: F840-F848

    • DOI

      10.1152/ajprenal.00511.2012

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi