Generation of new model mice that reflect human sporadic parkinson's disease

• Project/Area Number: 25860711
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Kyoto University
• Principal Investigator: ASANO Takeshi 京都大学, 医学(系)研究科(研究院), 研究員 (40649167)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: alpha-synuclein / 酸化ストレス / パーキンソン病 / αーシヌクレイン / α-シヌクレイン

Outline of Final Research Achievements

Parkinson’s disese is one of the common neurodegenerative diseases. The complete therapy of Parkinson’s disese has not been established because the pathogenesis of Parkinson’s disese is still unclear. To establish the new mammal models that resembles human Parkinson’s disese, we performed this study. We inter-crossed alpla-synuclein Tg mice with IRP2 Tg mice. The double-Tg mice had a little abnormal motor performance. Andn insoulble alpha-synuclein was obserbed in some regions in the brin of the double Tg mice. These results sugesest that our new mice is useful model for the reserch of Parkinson’s disese.

Research Products

• [Journal Article] Target- and input-dependent organization of AMPA and NMDA receptors in synaptic connections of the cochlear nucleus (2015)
  • Author(s): Rubio ME, Fukazawa Y, Kamasawa N, Clarkson C, Molnar E, Shigemoto R
  • Journal Title: J Comp Neurol Volume: 588 Pages: 29-35
  • DOI: 10.1016/j.neulet.2014.12.052
  • Peer Reviewed / Open Access
• [Journal Article] Immunoreactivity of valosin-containing protein in sporadic amyotrophic lateral sclerosis and in a case of its novel mutant (2014)
  • Author(s): Ayaki T, Ito H, Fukushima H, Inoue T, Kondo T, Ikemoto A, Asano T, Shodai A, Fujita T, Fukui S, Morino H, Nakano S, Kusaka H, Yamashita H, Ihara M, Matsumoto R, Kawamata J, Urushitani M, Kawakami H, Takahashi R.
  • Journal Title: Acta Neuropathol Commun. Volume: 2 Issue: 1 Pages: 172-185
  • DOI: 10.1186/s40478-014-0172-0
  • Peer Reviewed / Open Access
• [Presentation] Screening of small molecules that down-regulate α-synuclein expression (2015)
  • Author(s): Takeshi Asano
  • Organizer: Alpha-Synuclein: The Gateway to Parkinsonism
  • Place of Presentation: Innsbruck
  • Year and Date: 2015-02-11 – 2015-02-13
• [Presentation] パーキンソン病の治療に向けたα-シヌクレインの転写を抑制する低分子化合物のスクリーニング (2014)
  • Author(s): 浅野 剛史、山門 穂高、高橋 良輔
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Involvement of iron in the pathogenesis of Parkinson’s disease (2014)
  • Author(s): Takeshi Asano
  • Organizer: MDS-AOS Basic Scientists Summer School
  • Place of Presentation: 台北
  • Year and Date: 2014-07-13 – 2014-07-15
• [Presentation] マウスを用いたin vivoにおけるゾニサミドの作用機構 (2014)
  • Author(s): 浅野 剛史、山門 穂高、高橋 良輔
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] PINK1とParkinは脊椎動物において相補的にミトコンドリアを保護する (2013)
  • Author(s): 浅野 剛史、松井 秀彰、Robert Gavinio、上村 紀人、伊東 秀文、谷口 善仁、Jie Shen、武田 俊一、植村 健吾、山門 穂高、髙橋 良輔
  • Organizer: 第22回 日本Cell Death学会学術集会
  • Place of Presentation: 京都大学医学部 芝蘭会館
• [Presentation] マウスを用いたゾニサミドの作用機序の解析 (2013)
  • Author(s): 浅野 剛史、山門 穂高、髙橋 良輔
  • Organizer: 第7回 パーキンソン病・運動障害疾患コングレス
  • Place of Presentation: 京王プラザホテル
• [Presentation] PINK1とParkinは脊椎動物において相補的にミトコンドリアを保護する (2013)
  • Author(s): 浅野 剛史、松井 秀彰、Robert Gavinio、上村 紀人、伊東 秀文、谷口 善仁、Jie Shen、武田 俊一、植村 健吾、山門 穂高、髙橋 良輔
  • Organizer: 第36回 日本分子生物学会
  • Place of Presentation: 神戸国際展示場