The effects of dieatry yeasts on experimental autoimmune encephalomyelitis

• Project/Area Number: 25860712
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurology
• Research Institution: Osaka University
• Principal Investigator: TAKATA Kazushiro 大阪大学, 医学部附属病院, 医員 (20573223)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 多発性硬化症 / 腸管免疫 / 酵母 / 腸内細菌叢

Outline of Final Research Achievements

The intestinal microflora affects the pathogenesis of several autoimmune
diseases by influencing immune system function. We aimed to investigate the effects of various dietary yeasts contained in fermented foods on experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis (MS).
The effects of eight yeasts selected from 18 types of yeasts contained in fermented foods were examined. Of these, administration of Candida kefyr ameliorated the severity of EAE. Reduced numbers of inflammatory cells, and increased regulatory T cells and dendritic cells were observed. Analysis of microflora of C. kefyr-treated mice demonstrated decreased Bacteroides compared to control flora. Transfer of intestinal microbiota also resulted in decreased Bacteroides and ameliorated symptoms of EAE. Thus, oral administration of C. kefyr ameliorated EAE by altering the microflora. In addition, our findings also suggested the potential health benefits of dietary yeasts.

Research Products

• [Journal Article] Dietary Yeasts Reduce Inflammation in Central Nerve System via Microflora (2015)
  • Author(s): Takata K, Tomita T, Okuno T, Kinoshita M, Koda T, Honorat JA, Takei M, Hagihara K, Sugimoto T, Mochizuki H, Sakoda S, Nakatsuji Y
  • Journal Title: Annals of Clinical and Translational Neurology Volume: ２ Issue: 1 Pages: 56-66
  • DOI: 10.1002/acn3.153
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 11C-Acetate PET Imaging in Patients with Multiple Sclerosis. (2014)
  • Author(s): Takata K, Kato H, Shimosegawa E, Okuno T, Koda T, Sugimoto T, Mochizuki H, Hatazawa J, Nakatsuji Y.
  • Journal Title: PLoS One. Volume: 9 Issue: 11 Pages: e111598-e111598
  • DOI: 10.1371/journal.pone.0111598
  • Peer Reviewed / Open Access
• [Presentation] Intestinal microflora modified by Candida kefyr reduces the susceptibility to experimental autoimmune encephalomyelitis (2014)
  • Author(s): Takata K
  • Organizer: 2014 Joint ACTRIMS-ECTRIMS Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2014-09-10 – 2014-09-13
• [Presentation] 食餌成分（酵母）の腸管免疫を介した多発性硬化症病態への関与 (2014)
  • Author(s): 高田和城、中辻裕司、木下允、奥野龍禎、甲田亨、望月秀樹、佐古田 三郎
  • Organizer: 第26回神経免疫学会
  • Place of Presentation: 金沢市、石川県
  • Year and Date: 2014-09-04 – 2014-09-06
• [Presentation] 食餌成分（酵母）の腸管免疫を介した多発性硬化症病態への影響 (2014)
  • Author(s): 高田和城、中辻裕司、木下允、奥野龍禎、甲田亨、富田貴之、武井雅也、萩原幸一郎、佐古田 三郎、望月 秀樹
  • Organizer: 第55回日本神経学会学術大会
  • Place of Presentation: 福岡市、福岡県
  • Year and Date: 2014-05-21 – 2014-05-24
• [Presentation] Dietary Component, As An Environmental Factor, Affects The Development of EAE (2013)
  • Author(s): K. Takata, T. Tomita, T. Koda, T. Okuno, J.A. Honorat, M. Kinoshita, M. Takei, K. Hagihara, S. Sakoda, H. Mochizuki, Y. Nakatsuji
  • Organizer: PACTRIMS
  • Place of Presentation: Kyoto, Japan
• [Presentation] 食品中成分による多発性硬化症モデルの増悪 (2013)
  • Author(s): 高田 和城、Joseph A Honorat、甲田 亨、多田智、木下 允、奥野 龍禎、中辻 裕司、佐古田 三郎、望月 秀樹
  • Organizer: 日本神経学会
  • Place of Presentation: 東京