| Project/Area Number |
25860768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Endocrinology
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| Research Institution | Okayama University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | メラトニン / 下垂体 / BMP / 時計遺伝子 |
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| Outline of Final Research Achievements |
The role of melatonin, a regulator of circadian rhythm, in ACTH production by corticotrope cells has not been elucidated. We investigated the effect of melatonin on ACTH production in relation to the biological activity BMP-4 using mouse corticotrope AtT20 cells. Melatonin and BMP-4 activities were mutually augmented, leading to fine-tuning of ACTH production by corticotrope cells.
Next, we examined the correlation of mRNA levels of POMC and clock genes using mouse corticotrope AtT20 and rat lactosomatotrope GH3 cells. Collectively, the expressional correlation between POMC and Per and that between PRL and Clock were uncovered in the corticotrope and lactosomatotrope cells, respectively. Thus, the effects of melatonin and BMP-4 on the clock gene expression may imply the difference of stability of circadian fluctuations of ACTH and PRL secreted from the anterior pituitary.
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