| Project/Area Number |
25860781
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YOSHIMI Akihide 東京大学, 医学部附属病院, 助教 (80609016)
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| Research Collaborator |
TSUKAMOTO Ayato
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 骨髄異形成症候群 / 抵抗性メカニズム / 全エクソームシークエンシング / shRNAライブラリ / 耐性化メカニズム / アザシチジン |
|---|
| Outline of Final Research Achievements |
We conducted whole exome sequencing in patients with MDS and detected mutations specifically seen in azacitidine resistant phase, which were further narrowed down according to their functions and structures. Clone evolution during resistance acquisition was also analyzed with deep sequencing. Additionally, we screened responsible genes for azacitidine resistance using shRNA library system. We verified these candidate genes by demonstrating that suppression of the candidate genes conferred resistance to azacitidine in-vitro assays.
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