Identification of noncoding RNAs cooperating with Evi1

• Project/Area Number: 25860783
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: The University of Tokyo
• Principal Investigator: ARAI Shunya 東京大学, 医学部附属病院, 講師 (00579716)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 白血病 / Evi1 / lncRNA / EVI1

Outline of Final Research Achievements

Evi1 is one of epigenetic regulators, which is thought to work in cooperation with long noncoding RNA (lncRNA). I showed that Evi1 and a lncRNA, HOTAIR, have physical interaction in a leukemia cell line with high levels of Evi1 expression. I also revealed that a lncRNA, CRNDE, was overexpressed in immortalized hematopoietic cells with high levels of Evi1 expression. Those cells were phenotypically unchanged after knockdown of the expression of CRNDE.