Engraftment of Human Hematopoietic Stem Cells in Sheep after in Utero Transplantation

• Project/Area Number: 25860793
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: ABE Tomoyuki 自治医科大学, 医学部, 助教 (20610364)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 再生医学 / 幹細胞 / トランスレーショナルリサーチ / 移植・再生医療 / 応用動物

Outline of Final Research Achievements

The generation of definitive (engraftable) human hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) has been a major challenge in hematology. We are trying to generate hematopoietic chimera in sheep with human iPSCs.

Research Products

• [Journal Article] A long-term follow-up study on the engraftment of human hematopoietic stem cells in sheep. (2014)
  • Author(s): Tomoyuki Abe, Yutaka Hanazono, Yoshikazu Nagao
  • Journal Title: Experimental Animals Volume: 63 Issue: 99999 Pages: 475-481
  • DOI: 10.1538/expanim.63.475
  • NAID: 130004701642
  • Peer Reviewed / Open Access
• [Journal Article] MHC-matched induced pluripotent stem cells can attenuate cellular and humoral immune responses but are still susceptible to innate immunity. (2014)
  • Author(s): Mizukami Y, Abe T, Shibata H, Makimura Y, Fujishiro SH, Yanase K, Hishikawa S, Kobayashi E and Hanazono Y
  • Journal Title: PLoS One Volume: 9 Issue: 6 Pages: e98319-e98319
  • DOI: 10.1371/journal.pone.0098319
  • Peer Reviewed / Open Access
• [Presentation] ヒツジ子宮内移植系におけるヒト造血細胞の生着促進・増幅技術の開発 (2015)
  • Author(s): 阿部朋行、長尾慶和、原明日香、スブド・ビャンバー、柳瀬公秀、ボラジギン・サラントラガ、緒方和子、山口美緒、福森理加、花園豊
  • Organizer: 第17回日本異種移植研究会
  • Place of Presentation: 下野市
  • Year and Date: 2015-03-14
• [Presentation] 大動物を用いた再生医療研究～ヒト血液細胞をもつヒツジの作出 (2015)
  • Author(s): 阿部朋行
  • Organizer: 第18回予防衛生協会セミナー
  • Place of Presentation: つくば市
  • Year and Date: 2015-02-14
  • Invited
• [Presentation] Hematopoietic Engraftment of Human iPS Cells in Sheep after in Utero Transplantation. (2014)
  • Author(s): Tomoyuki Abe, Suvd Byambaa, Kimihide Yanase, Asuka Hara, Yoshikazu Nagao and Yutaka Hanazono.
  • Organizer: 第13回 自治医科大学シンポジウム
  • Place of Presentation: 下野市
  • Year and Date: 2014-09-05
• [Presentation] ヒツジ子宮内移植系におけるヒト造血細胞の生着・増幅技術の開発 (2014)
  • Author(s): 阿部朋行、長尾慶和、柳瀬公秀、原明日香、ボラジギン・サラントラガ、緒方和子、山口美緒、花園豊
  • Organizer: 第61回日本実験動物学会総会
  • Place of Presentation: 札幌
  • Year and Date: 2014-05-15 – 2014-05-17
• [Presentation] ヒツジ子宮内移植系によるヒト造血幹細胞の定量評価の試み (2014)
  • Author(s): 阿部朋行、長尾慶和、柳瀬公秀、サラントラガ、緒方和子、山口美緒、花園豊
  • Organizer: 第36回 日本造血細胞移植学会
  • Place of Presentation: 沖縄
• [Presentation] ヒツジ子宮内異種移植(II)：長期間の造血再構築 (2013)
  • Author(s): 阿部朋行、長尾慶和、柳瀬公秀、サラントラガ、緒方和子、山口美緒、林聡、花園豊
  • Organizer: 第16回日本異種移植研究会
  • Place of Presentation: 大阪
• [Presentation] Long-term comparative study on the engraftment of human hematopoietic stem cells in sheep after xenogeneic in utero transplantation. (2013)
  • Author(s): Tomoyuki Abe, Shigeo Masuda, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono.
  • Organizer: The 12th Congress of the International Xenotransplantation Association
  • Place of Presentation: Osaka
• [Presentation] ヒト化ヒツジの作製を目指して (2013)
  • Author(s): 阿部朋行、花園豊
  • Organizer: 第60回日本実験動物学会総会
  • Place of Presentation: つくば
  • Invited
• [Presentation] ヒツジ子宮内異種移植(I)：生着条件の検討 (2013)
  • Author(s): 長尾慶和、阿部朋行、柳瀬公秀、サラントラガ、緒方和子、山口美緒、林聡、花園豊
  • Organizer: 第16回日本異種移植研究会
  • Place of Presentation: 大阪
• [Presentation] Factors influencing engraftment of monkey embryonic stem cells in sheep after xenogeneic in utero transplantation. (2013)
  • Author(s): Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Borjigin Sarentonglaga, Kazuko Ogata, Mio Yamaguchi, Satoshi Hayashi, Yoshihiro Kitano and Yutaka Hanazono.
  • Organizer: The 12th Congress of the International Xenotransplantation Association
  • Place of Presentation: Osaka