• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Generation and characterization of homozygous plasminogen-Tochigi mutant mice bearing reduced fibrinolytic activity

Research Project

Project/Area Number 25860801
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionOsaka University (2014)
National Cardiovascular Center Research Institute (2013)

Principal Investigator

TASHIMA Yuko  大阪大学, 微生物病研究所, 助教 (10423104)

Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsプラスミノーゲン / 線溶 / 静脈血栓症 / 肺塞栓 / プラスミノーゲン栃木変異
Outline of Final Research Achievements

Plasminogen (Plg) is a precursor of a serine protease, plasmin to degrade fibrin fiber in blood clot. Plg-A620T mutation previously named as Plg-Tochigi was identified in Japanese patients with deep venous thrombosis. We investigated whether the homozygous Plg-A622T mutation corresponding to human Plg-Tochigi caused serious thrombosis in mice. Compared to wild-type mice, Plg-A620T mice showed the lower activity of plasmin, but did not affect wound healing unlike Plg-KO mice. Plg-Tochigi mutation did not significantly affect the severity of thrombogenesis under three different experimental thrombosis models: a middle cerebral artery occlusion model of ischemia-reperfusion injury, acute pulmonary embolism model by injection of recombinant human tissue factor via inferior vena cava, and inferior vena cava thrombosis model with electrolytic stimulation. In conclusion, homozygous Plg-Tochigi mutation showed reduced enzymatic activity of plasmin but did not cause severe thrombosis in mice.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (6 results) Book (3 results)

  • [Journal Article] Influence of ADAMTS13 deficiency on venous thrombosis in mice2015

    • Author(s)
      Tashima Y, Banno F, Akiyama M, Miyata T
    • Journal Title

      Thromb Haemost

      Volume: 印刷中 Issue: 07 Pages: 206-207

    • DOI

      10.1160/th14-08-0656

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 日本人に高頻度に見られるプラスミノーゲン栃木変異をもつ遺伝子改変マウスの血栓傾向の解析2014

    • Author(s)
      田嶌優子, 坂野史明, 喜多俊行, 松田泰幸, 柳本広二, 宮田敏行
    • Organizer
      第19回日本病態プロテアーゼ学会学術集会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府豊中市)
    • Year and Date
      2014-08-08
    • Related Report
      2014 Annual Research Report
  • [Presentation] ADAMTS13遺伝子欠損マウスの静脈血栓塞栓症状の解析2014

    • Author(s)
      田嶌優子, 坂野史明, 宮田敏行
    • Organizer
      第36回日本血栓止血学会学術集会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2014-05-29 – 2014-05-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Generation and characterization of homozygous plasminogen-Tochigi mutant mice bearing reduced fibrinolytic activity2014

    • Author(s)
      Yuko Tashima, Fumiaki Banno, Toshiyuki Kita, Yasuyuki Matsuda, Hiroji Yanamoto, Toshiyuki Miyata
    • Organizer
      Gordon Research Seminar, Plasminogen Activation & Extracellular Proteolysis
    • Place of Presentation
      Four Points Sheraton / Holiday Inn Express (USA, California, Ventura)
    • Related Report
      2013 Research-status Report
  • [Presentation] Generation and characterization of homozygous plasminogen-Tochigi mutant mice bearing reduced fibrinolytic activity2014

    • Author(s)
      Yuko Tashima, Fumiaki Banno, Toshiyuki Kita, Yasuyuki Matsuda, Hiroji Yanamoto, Toshiyuki Miyata
    • Organizer
      Gordon Research Conferences, Plasminogen Activation & Extracellular Proteolysis
    • Place of Presentation
      Four Points Sheraton / Holiday Inn Express (USA, California, Ventura)
    • Related Report
      2013 Research-status Report
  • [Presentation] Generation and characterization of homozygous plasminogen-Tochigi mutant mice bearing reduced fibrinolytic activity2013

    • Author(s)
      Yuko Tashima, Fumiaki Banno, Toshiyuki Kita, Yasuyuki Matsuda, Hiroji Yanamoto, Toshiyuki Miyata
    • Organizer
      XXIV Congress of the International Society on Thrombosis and Haemostasis
    • Place of Presentation
      Amsterdam RAI Exhibition and Convention Centre (The Netherlands, Amsterdam)
    • Related Report
      2013 Research-status Report
  • [Presentation] マウスプラスミノーゲン栃木変異は血漿プラスミン活性を低下させるが血栓塞栓症の原因にはならない

    • Author(s)
      Yuko Tashima, Fumiaki Banno, Toshiyuki Kita, Yasuyuki Matsuda, Hiroji Yanamoto, Toshiyuki Miyata
    • Organizer
      第86回日本生化学会大会
    • Place of Presentation
      パシフィコ横浜 (神奈川県横浜市)
    • Related Report
      2013 Research-status Report
  • [Book] 新・血栓止血学, 2凝固反応, (2部ショック・敗血症と凝血・炎症系, 19.凝固系と補体系反応)2015

    • Author(s)
      田嶌優子, 木下タロウ,一瀬白帝, 他
    • Publisher
      金芳堂
    • Related Report
      2014 Annual Research Report
  • [Book] プリンシプル血液疾患の臨床, よくわかる血栓・止血異常の診療, (第1章 血栓止血異常症を理解するために, 凝固反応を理解する)2014

    • Author(s)
      宮田敏行, 田嶌優子, 金倉譲, 他
    • Total Pages
      297
    • Publisher
      中山書店
    • Related Report
      2014 Annual Research Report
  • [Book] シリーズ  プリンシプル血液疾患の臨床、 4. 血栓・止血異常の診察、 第1章 血栓止血異常症を理解するために、  B. 凝固反応を理解する2014

    • Author(s)
      宮田敏行 田嶌優子
    • Publisher
      中山書店
    • Related Report
      2013 Research-status Report

URL: 

Published: 2014-07-25   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi