A novel treatment strategy aiming joint regeneration of rheumatoid arthritis and osteoarthritis by mesenchymal stem cells with nano-fiber scaffold

• Project/Area Number: 25860821
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Collagenous pathology/Allergology
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: SONOMOTO Koshiro 産業医科大学, 医学部, 非常勤医師 (20636914)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 関節再生 / 関節リウマチ / スキャフォールド / 変形性関節症 / 再生医療 / 間葉系幹細胞

Outline of Final Research Achievements

Mesenchymal stem cells (MSCs) have immunosuppressive activity and can differentiate into bone and cartilage; and thus seem ideal for treatment of rheumatoid arthritis (RA) and osteoarthritis (OA). Here, we investigated the immunosuppressive potential, osteogenesis and chondrogenesis potentials of MSCs seeded onto nano-fiber scaffolds (NFs). Healthy donor derived human MSC seeded on NF sheet (NF-MSC) successfully suppressed the collagen induced arthritis of rats. In this context, NFs enhanced the ability of MSCs of cytokines production. Next, in vitro experiment of NF-MSC revealed that NFs stimulated the differentiation of MSCs derived from healthy as well as RA- or OA-patients into osteoblasts, chondrocytes and osteocytes. These data show the possible use of NF-MSCs for the repair of RA-affected joints.

Research Products

• [Journal Article] Spontaneous differentiation of human mesenchymal stem cells on poly-lactic-co-glycolic acid nano-fiber scaffold (2016)
  • Author(s): Koshiro Sonomoto, Kunihiro Yamaoka, Hiroaki Kaneko, Kaoru Yamagata, Kei Sakata, Xiangmei Zhang, Masahiro Kondo, Yukichi Zenke, Ken Sabanai, Shingo Nakayamada, Akinori Sakai, Yoshiya Tanaka
  • Journal Title: PLOS ONE Volume: in press Issue: 4 Pages: e0153231-e0153231
  • DOI: 10.1371/journal.pone.0153231
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Local Delivery of Mesenchymal Stem Cells with Poly-Lactic-Co-Glycolic Acid Nano-Fiber Scaffold Suppress Arthritis in Rats (2014)
  • Author(s): Xiangmei Zhang, Kunihiro Yamaoka, Koshiro Sonomoto, Hiroaki Kaneko, Makoto Satake, Yuka Yamamoto, Masahiro Kondo, Jidong Zhao, Ippei Miyagawa, Kaoru Yamagata, Shunsuke Fukuyo, Yosuke Okada, Yoshiya Tanaka
  • Journal Title: ＰＬＯＳＯＮＥ Volume: - Issue: 12 Pages: e114621-e114621
  • DOI: 10.1371/journal.pone.0114621
  • Peer Reviewed / Open Access
• [Journal Article] The non-canonical Wnt5a/Ror2 signaling pathway in bone metabolism (2014)
  • Author(s): Koshiro Sonomoto, Kunihiro Yamaoka and Yoshiya Tanaka
  • Journal Title: Inflammation and Regeneration Volume: 34 Pages: 103-108
  • NAID: 130004482325
  • Peer Reviewed
• [Presentation] IL-17によるヒト間葉系幹細胞の軟骨細胞分化抑制作用の解析 (2013)
  • Author(s): 近藤正弘、山岡邦宏、園本格士朗、福與俊介、尾下浩一、岡田洋右、齋藤和義、田中良哉
  • Organizer: 第57回日本リウマチ学会
  • Place of Presentation: 京都国際会館