Elucidating mechanisms for coupling bone matrix formation with mineralization
Project/Area Number |
25860858
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Pediatrics
|
Research Institution | Osaka University |
Principal Investigator |
Kubota Takuo 大阪大学, 医学(系)研究科(研究院), 助教 (40629135)
|
Research Collaborator |
Fujiwara Makoto 大阪大学, 歯学部附属病院, 助教 (50625697)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 小児科学 / 骨代謝学 / 骨粗鬆症 / スクレロスチン / くる病 / 線維芽細胞 / 骨細胞 / 転写因子 / 骨石灰化 / Wntシグナル |
Outline of Final Research Achievements |
Sclerostin, encoded by SOST, is a secretory protein that suppresses osteogenesis by inhibiting Wnt signaling. However, the regulatory mechanism underlying SOST expression remains unclear. We identified four transcription factors, ATF3, KLF4, PAX4, and SP7 that induced SOST expression and sclerostin secretion in human dermal fibroblasts. Parathyroid hormone suppressed the induced SOST and sclerostin, whereas hypoxia and prostaglandin E2 increased the induced SOST. This model may contribute to elucidating the regulatory mechanisms underlying SOST expression and advancing drug development for metabolic bone diseases.
|
Report
(4 results)
Research Products
(9 results)
-
[Journal Article] Successful induction of sclerostin in human-derived fibroblasts by 4 transcription factors and its regulation by parathyroid hormone, hypoxia, and prostaglandin E2.2016
Author(s)
Fujiwara M, Kubota T, Wang W, Ohata Y, Miura K, Kitaoka T, Okuzaki D, Namba N, Michigami T, Kitabatake Y, Ozono K.
-
Journal Title
Bone
Volume: 85
Pages: 91-98
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Two Japanese familial cases of Caffey disease with and without the common COL1A1 mutation and normal bone density, and review of the literature.2014
Author(s)
Taichi Kitaoka, Yoko Miyoshi, Noriyuki Namba, Kohji Miura, Takuo Kubota, Yasuhisa Ohata, Makoto Fujiwara, Masaki Takagi, Tomonobu Hasegawa, Harald Jüppner, Keiichi Ozono
-
Journal Title
European Journal of Pediatrics
Volume: [Epub ahead of print]
Issue: 6
Pages: 1-6
DOI
Related Report
Peer Reviewed
-
[Journal Article] Serum Fibroblast Growth Factor 23 Is a Useful Marker to Distinguish Vitamin D-Deficient Rickets from Hypophosphatemic Rickets.2014
Author(s)
Kubota T, Kitaoka T, Miura K, Fujiwara M, Ohata Y, Miyoshi Y, Yamamoto K, Takeyari S, Yamamoto T, Namba N, Ozono K.
-
Journal Title
Hormone Research in Paediatrics
Volume: [Epub ahead of print]
Issue: 4
Pages: 1-7
DOI
Related Report
Peer Reviewed
-
-
-
-
-