| Project/Area Number |
25860861
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kobe University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
IIJIMA Kazumoto (HARA Shigeo / KAITO Hiroshi / NOZU Kandai) 神戸大学, 大学院医学研究科, 教授 (00240854)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 溶血性尿毒症症候群 / 志賀毒素 / アポトーシス / サイトカイン / 尿細管培養細胞 / ベロ毒素 |
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| Outline of Final Research Achievements |
The purpose of this study is to reveal the mechanism of tubular injury with diarrhea associated hemolytic uremic syndrome (D+HUS). At first, we gave Shiga toxin (ST) 1, and 2 to human tubular cultured cell to examine the maximal concentration and duration of toxin. As the result, 10*-7 in 24 hours of ST was most suitable condition with maximal apoptosis. Second, messenger RNA of apoptosis related protein was analyzed with array PCR method. However, few significant factors of apoptosis was not identified with the definite pathway of apoptosis. Same results was found in serum of patients with D+HUS. Third, we examine cytokines which developed during tubular injury. In our assay, there were no significant cytokines. Thus, our strategy or our assay were difficult to reveal tubular injury.
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