| Project/Area Number |
25860939
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | ultraviolet radiation, / alkannin / apoptosis / HSP70 / keratinocytes / ultraviolet / keratinocute / Alkannin / アポトーシス / UVB / アルカンニン |
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| Outline of Final Research Achievements |
Alkannin is an active constituent from the root extract of Alkanna tinctoria of the Boraginaceae family and it may have utility as HSP70 inducer in living organisms. Here, the effects of alkannin-induced HSP70 on UVB (40 mJ/cm2)-induced apoptosis were investigated in human keratinocyte HaCaT cells. Pretreatment of the cells with alkannin (1 M) caused significant inhibition of UVB-induced apoptosis in HaCaT cells. Moreover, pretreatment with alkannin decreased UVB-induced cleavage of caspase-3. On the other hand, the stimulation of HSP70 inhibitor (KNK437) increased UVB-induced apoptosis in a dose-dependent manner. In addition, differences in gene expression were investigated using a genechip, associated with the suppression of UVB-induced apoptosis in presence of alkannin. The results indicate that alkannin suppress UVB-induced apoptosis via the induction of HSP70 in human keratinocytes, and therefore, this result suggests the usefulness of the compound as antiaging agent.
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