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Evaluation of percutaneous immune responses and role of natural moisturizing factor in filaggrin monomer transgenic mice

Research Project

Project/Area Number 25860944
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionHamamatsu University School of Medicine

Principal Investigator

IKEYA Shigeki  浜松医科大学, 医学部附属病院, 助教 (40436936)

Co-Investigator(Kenkyū-buntansha) TOKURA Yoshiki  浜松医科大学, 医学部, 教授 (00172156)
SAKABE Jun-ichi  浜松医科大学, 医学部, 特任助教 (30631494)
Research Collaborator 高橋 慶人  株式会社カネボウ化粧品, 主任研究員
行 卓男  株式会社カネボウ化粧品, 研究員
Project Period (FY) 2013-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsフィラグリン / 天然保湿因子 / アトピー性皮膚炎 / 皮膚バリア / 接触性皮膚炎 / 皮膚バリア機能 / 表皮バリア
Outline of Final Research Achievements

We generated a FLG monomers transgenic mouse under the control of involucrin promoter. The FLG-TG mouse could have more filaggrin monomers and its derivatives, NMF. We speculate that our mice are more protective against outer stimuli and are prone to develop both irritant and allergic dermatitis. This type of mice revealed decreased transepidermal water loss and prevent the out-to-inside transpenetration of a low molecular weight chromatophore, Lucifer yellow. These findings clearly indicate the importance of filaggrin monomers in skin barrier. On the other hand, SC hydration was not up-regulated in FLG-TG mice. The amount of NMF, which could be expected to correlate with SC hydration, is dependent on proteases including bleomycin hydrolase. Thus, we thought that the amount of NMF in our transgenic mice need careful assessment. In the future plan, we would like to analyze FLG-TG mice mainly focusing on the immunological reaction.

Report

(3 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2014 2013

All Journal Article (4 results) (of which Peer Reviewed: 3 results,  Open Access: 1 results)

  • [Journal Article] Proteome analysis of stratum corneum from atopic dermatitis patients by hybrid quadrupole-orbitrap mass spectrometer2014

    • Author(s)
      Jun-Ichi Sakabe, Koji Kamiya, Hayato Yamaguchi, Shigeki Ikeya, Takahiro Suzuki, Masahiro Aoshima, Kazuki Tatsuno, Toshiharu Fujiyama, Masako Suzuki, Tsuyoshi Yatagai, Taisuke Ito, Toshiyuki Ojima, Yoshiki Tokura
    • Journal Title

      J Allergy Clin Immunol

      Volume: 134 Issue: 4 Pages: 957-960

    • DOI

      10.1016/j.jaci.2014.07.054

    • NAID

      40020962750

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] アトピー性皮膚炎患者におけるフィラグリン遺伝子変異と手掌皺亢進・尋常性魚鱗癬との関連.2014

    • Author(s)
      坂部 純一、 山口 隼人、 福家 辰樹、 池谷 茂樹、 神谷 浩二、 龍野 一樹、 戸倉 新樹
    • Journal Title

      日本小児皮膚科学会雑誌

      Volume: 33 Pages: 127-131

    • Related Report
      2014 Annual Research Report
  • [Journal Article] First Japanese case documenting GJB3 mutation2013

    • Author(s)
      Shigeki Ikeya
    • Journal Title

      Journal of Dermatology

      Volume: 40 Pages: 402-403

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Atopic dermatitis presenting as generalized poikiloderma with filaggrin gene mutation2013

    • Author(s)
      Shinsuke Nakazawa
    • Journal Title

      Journal of Dermatology

      Volume: 41 Issue: 3 Pages: 230-231

    • DOI

      10.1111/1346-8138.12339

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

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Published: 2014-07-25   Modified: 2019-07-29  

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