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Establishment of domain specific Enzyme-Linked ImmunoSorbent Assay for pemphigus using recombinant proteins expressed by mammalian cells.

Research Project

Project/Area Number 25860973
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionKurume University

Principal Investigator

OHYAMA Bungo  久留米大学, 医学部, 講師 (90461441)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords自己免疫性水疱症 / エピトープ解析 / ドメイン特異的ELISA法 / 哺乳類細胞発現系組み換え蛋白質 / 昆虫細胞発現系組み換え蛋白質 / 免疫沈降法 / 免疫ブロット法 / ELISA法 / バキュロウイルス発現系 / 哺乳細胞発現系
Outline of Final Research Achievements

Pemphigus is autoimmune blistering disease caused by autoantibodies. Autoantibodies react with extracellular domains of desmoglein (Dsg) 1, Dsg3, desmocollin 1, and Dsc3 as antigens, and then cause blisters by abolished the adhesion between Dsg and Dsc proteins.
We attempted to establish domain specific Enzyme-Linked ImmunoSorbent Assay for pemphigus using recombinant proteins expressed by mammalian cells, because recombinant proteins of mammalian expression system keeps higher-order structures of protein than baculovirus insect expression system. We released some interesting data about epitopes which autoantibodies reacted against Dsg EC domains in three reports, and have established of IgA ELISAs of mammalian recombinant proteins of human Dsc 1-3.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (4 results)

All 2016 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results)

  • [Journal Article] Establishment of IgA ELISAs of mammalian recombinant proteins of human desmocollins 1-3.2016

    • Author(s)
      eye K, Numata S, Ohzono A, Ohyama B, Tsuchisaka A, Koga H, Hachiya T, Tsuruta D, Ishii N, Hashimoto T
    • Journal Title

      J Dermatol Sci

      Volume: 印刷中 Issue: 1 Pages: 75-77

    • DOI

      10.1016/j.jdermsci.2016.04.001

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Clinical and immunological profiles in 17 Japanese patients with drug-induced pemphigus studied at Kurume University.2014

    • Author(s)
      Yoshimura K, Ishii N, Hamada T, Abe T, Ono F, Hashikawa K, Fukuda S, Ohyama B, Koga H, Sogame R, Teye K, Ochiai T, Nakajima H, Nakajima K, Iijima S, Kanzaki M, Kojima K, Nagatani T, Fujimoto W, Karashima T, Nakama T, Ohata C, Furumura M, Tsuruta D, Hashimoto T
    • Journal Title

      Br J Dermatol

      Volume: 171 Issue: 3 Pages: 544-553

    • DOI

      10.1111/bjd.12925

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Detection of Autoantibodies to Precursor Proteins of Desmogleins in Sera of a Patient with Bowen Carcinoma2014

    • Author(s)
      Demitsu T, Yamada T, Nakamura S, Kakurai M, Dohmoto T, Kamiya K, Aoyama Y, Iwatsuki K, Yamagami J, Ohyama B, Ohata C, Koga H, Hashimoto T
    • Journal Title

      Acta Derm Venereol

      Volume: 94 Issue: 5 Pages: 601-603

    • DOI

      10.2340/00015555-1776

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Antibodies to the amino-terminal domain of desmoglein 1 are retained during transition from pemphigus vulgaris to pemphigus foliaceus.2014

    • Author(s)
      Espana A, Koga H, Suarez-Fernandez R, Ohata C, Ishii N, Irarrazaval I, Teye K, Ohyama B, Hashimoto T
    • Journal Title

      Eur J Dermatol

      Volume: in press

    • Related Report
      2013 Research-status Report
    • Peer Reviewed

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Published: 2014-07-25   Modified: 2019-07-29  

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