Identification of gene mutation profiles related to responses to radiation therapy

• Project/Area Number: 25861066
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Gunma University
• Principal Investigator: Oike Takahiro 群馬大学, 医学部附属病院, 助教 (10643471)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 遺伝子変異 / 放射線感受性 / 放射線感受性遺伝子 / 子宮頸癌 / 放射線治療 / 遺伝子異常

Outline of Final Research Achievements

The present study aimed to identify gene mutation profiles in human cancer that correlate with sensitivity to radiation therapy. From a prospective and retrospective cohort of patients with uterine cervical cancer treated by radiation therapy, we obtained tumor biopsy samples before initiation of radiation therapy. We extracted DNA from the formalin-fixed and paraffin-embedded tumor biopsy samples. Using a next-generation sequencer, we performed target-capture sequencing of the extracted DNA and analyzed mutational status in 50 known cancer-related genes. Consequently, we identified several genes including FGFR family genes and SMAD4 whose mutational status can affect sensitivity to radiation therapy.

Research Products

• [Journal Article] The EGFR mutation status affects the relative biological effectiveness of carbon-ion beams in non-small cell lung carcinoma cells. (2015)
  • Author(s): Amornwichet N, Oike T, Shibata A, Nirodi CS, Ogiwara H, Makino H, Kimura Y, Hirota Y, Isono M, Yoshida Y, Ohno T, Kohno T, Nakano T.
  • Journal Title: Sci Rep. Volume: 11 Issue: 1 Pages: 11305-11305
  • DOI: 10.1038/srep11305
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Polymyositis and fatal interstitial pneumonia following pelvic irradiation that led to unexpectedly severe adverse effects: a case report. (2015)
  • Author(s): Oike Takahiro, Ohno Tatsuya, Shirai Katsuyuki, Inoue Takashi, Nakano Takashi.
  • Journal Title: Clinical Case Reports Volume: 8 Issue: 8 Pages: 710-713
  • DOI: 10.1002/ccr3.322
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromatin-regulating proteins as targets for cancer therapy. (2014)
  • Author(s): Oike T, Ogiwara H, Amornwichet N, Nakano T, Kohno T.
  • Journal Title: J Radiat Res Volume: 55 Issue: 4 Pages: 613-628
  • DOI: 10.1093/jrr/rrt227
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Carbon-Ion Beam Irradiation Kills X-Ray-Resistant p53-Null Cancer Cells by Inducing Mitotic Catastrophe (2014)
  • Author(s): Amornwichet N, Oike T, Shibata A, Ogiwara H, Tsuchiya N, Yamauchi M, Saitoh Y, Sekine R, Isono M, Yoshida Y, Ohno T, Kohno T, Nakano T
  • Journal Title: PLoS One Volume: 9 Issue: 12 Pages: e115121-e115121
  • DOI: 10.1371/journal.pone.0115121
  • NAID: 120006986291
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Chromatin-regulating proteins as targets for cancer therapy (2014)
  • Author(s): Takahiro Oike, Hideaki Ogiwara, Napapat Amornwichet, Takashi Nakano, Takashi Kohno
  • Journal Title: Journal of Radiation Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Inactivating mutations in SWI/SNF chromatin remodeling genes in human cancer. (2013)
  • Author(s): Oike T, Ogiwara H, Nakano T, Kohno T
  • Journal Title: Jpn J Clin Oncol Volume: 43 Issue: 9 Pages: 849-855
  • DOI: 10.1093/jjco/hyt101
  • Peer Reviewed
• [Presentation] Carbon-ion beams efficiently kill cancer cells showing resistance to X-rays and cisplatin by inducing mitotic catastrophe. (2015)
  • Author(s): Kobayashi Daijiro, Oike Takahiro, Shibata Atsushi, Niimi Atsuko, Amornwichet Napapat, Yoshimoto Yuya, Sato Hiro, Isono Mayu, Yoshida Yukari, Kohno Takashi, Ohno Tatsuya, Nakano Takashi.
  • Organizer: 日本放射線腫瘍学会第28回学術大会
  • Place of Presentation: ベイシア文化ホール（群馬県前橋市）
  • Year and Date: 2015-11-19
• [Presentation] がんの遺伝的多様性に基づく分子標的と増感を目指したトランスレーショナル・リサーチ (2015)
  • Author(s): 尾池貴洋，荻原秀明，柴田淳史，水上達治，河野隆志，中野隆史.
  • Organizer: 第17回癌治療増感研究シンポジウム
  • Place of Presentation: 奈良県民文化会館（奈良県奈良市）
  • Year and Date: 2015-02-06 – 2015-02-07
  • Invited
• [Presentation] Carbon-ion beam irradiation kills X-ray-resistant p53-null cancer cells by inducing mitotic catastrophe (2014)
  • Author(s): 尾池貴洋, ナパパ・アモウィチェト, 柴田淳史, 荻原秀明, 磯野真由, 吉田由香里, 大野達也, 河野隆志, 中野隆史.
  • Organizer: 日本放射線影響学会第57回大会
  • Place of Presentation: かごしま県民交流センター（鹿児島県鹿児島市）
  • Year and Date: 2014-10-01 – 2014-10-03
• [Presentation] Evaluation of DNA damage response after carbon-ion beam irradiation in p53-mutant cancer cells (2014)
  • Author(s): Amornwichet N, Oike T, Komachi M, Isono M, Ogiwara H, Kohno T, Nakano T.
  • Organizer: ASTRO’s 56th Annual Meeting.
  • Place of Presentation: San Francisco (USA)
  • Year and Date: 2014-09-14 – 2014-09-17
• [Presentation] Carbon-ion beam irradiation kills X-ray-resistant p53-null cancer cells by inducing mitotic catastrophe (2014)
  • Author(s): ナパパ・アモウィチェト，尾池貴洋，柴田淳史，荻原秀明，木村由夏，磯野真由，吉田由加里，大野達也，河野隆志，中野隆史．
  • Organizer: 第43回放射線による制癌シンポジウム・第52回生物部会学術大会
  • Place of Presentation: メルパルク京都（京都府京都市）
  • Year and Date: 2014-07-11 – 2014-07-12