Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lung adenocarcinoma
Project/Area Number |
25861071
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Radiation science
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Research Institution | Gunma University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | ヒト肺腺癌細胞 / 炭素線照射 / 遊走能 / Rho / ROCK / ヒト肺線癌細胞 |
Outline of Final Research Achievements |
This study aimed to investigate the effect of carbon ion (C-ion) irradiation on cell motility through the Rho signaling pathway in the human lung adenocarcinoma cell line A549. At 48 h after irradiation, the wound-healing assay demonstrated that migration was significantly greater in cells irradiated with C-ion than in unirradiated cells. Similarly, F-actin staining showed that the formation of protrusions was significantly increased in cells irradiated with C-ion compared with unirradiated cells. Western-blot analysis showed that C-ion irradiation increased P-MLC2 expression compared with in unirradiated controls. Exposure to a non-toxic concentration of Y-27632, a specific inhibitor of ROCK, reduced the expression of P-MLC2 after C-ion irradiation, resulting in a significant reduction in migration. These data suggest that C-ion irradiation increases cell motility in A549 cells via the Rho signaling pathway and that ROCK inhibition reduces that effect.
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Report
(4 results)
Research Products
(3 results)
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[Journal Article] Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-27632 in lungadenocarcinoma A549 cells.2014
Author(s)
Kazutoshi Murata, Shin-ei Noda, Takahiro Oike, Akihisa Takahashi, Yukari Yoshida, Yoshiyuki Suzuki, Tatsuya Ohno, Tomoo Funayama, Yasuhiko Kobayashi, Takeo Takahashi, Takashi Nakano
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Journal Title
J Radiat Res.
Volume: 55
Issue: 4
Pages: 658-664
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] Increase in cell motility by carbon ion irradiation via the Rho signaling pathway and its inhibition by the ROCK inhibitor Y-276322015
Author(s)
Kazutoshi MURATA, Shin-ei NODA, Takahiro OIKE, Akihisa TAKAHASHI, Yukari YOSHIDA, Yoshiyuki SUZUKI, Tatsuya OHNO, Tomoo FUNAYAMA, Yasuhiko KOBAYASHI, Takeo TAKAHASHI, Takashi NAKANO
Organizer
15th International Congress of Radiation Research
Place of Presentation
Kyoto,Japan
Year and Date
2015-05-25
Related Report
Int'l Joint Research
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