| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Radiation cancer therapy is now one of the most successful method due technological innovations of the highly accurate and selective localization of the dose. The purpose of this study was to construct a simple model in radiotherapy, and to clarify the effect and its mechanism of cellular communication between irradiated cancer cells and non-irradiated normal cells. We applied microbeam irradiation technologies to target only the A549 human lung carcinoma cells in the mixed population of cells with human normal lung fibroblast cells. SPICE-NIRS microbeam was used to irradiate 500 protons of 3.4 MeV (LET: 12 Kiev/um in water) two nuclei of A549 cells. As a result, we found that two major pathways of radiation induced bystander response, media transfer signaling pathway and gap junction mediated signaling pathway mediate DNA double strand breaks repair pathway in irradiated A549 cells by non-irradiated WI-38 cells.
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