| Project/Area Number |
25861141
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
Sato Katsutoshi 国立研究開発法人放射線医学総合研究所, 重粒子医科学センター, 研究員 (20589650)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 粒子線治療 / 炭素イオン線抵抗性 / 炭素イオン線治療 / 放射線抵抗性がん細胞 / ヘテロクロマチン |
|---|
| Outline of Final Research Achievements |
Radioresistant cancer cell line X60 and C30 was originally established by means of repeated exposure the mouse squamous cell carcinoma cell line NR-S1 to X-ray and carbon ion beam (C-ion) irradiation, respectively. The results showed that the X60 cells acquired the resistance to both X-ray and C-ion irradiation. On the other hand, the X-ray and C-ion sensitivity of C30 cells was approximately same as that of NR-S1 cells. Moreover, it is shown that the X-ray and C-ion resistance was strongly correlated with the heterchromatin domain number, and the Rad51 focus formation was significantly promoted in X-ray and C-ion resistant X60 cells compared with that in NR-S1 and C30 cells. Our results suggested that homologous recombination repair in heterochromatin region is associated with the X-ray and C-ion resistance in cancer cells.
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