Methodology of the protein expression in the cardiac graft outside the body

• Project/Area Number: 25861147
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hokkaido University
• Principal Investigator: Wakayama Kenji 北海道大学, 大学病院, 医員 (50646544)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 移植・再生医療 / 虚血再灌流 / 臓器保存 / 酸化ストレス / 抗酸化 / 心移植 / シャペロン / 重水 / 心臓

Outline of Final Research Achievements

To enlarge donor pool for the heart transplantation, reliable method to repair the "less-than-ideal grafts". In the present study, we evaluated the merit and demerit of the oxidative phosphorylation under hypothermia. The results are summarized as follows. 1) Oxidative phosphorylation is progressed under hypothermic conditions. 2) The energy source of the ATP production under hypothermic oxygenated conditions is endogenous amino acids derived from the autophagic pathway. 3) This oxidative phosphorylation under hypothermia worsen the cellular death due to the oxidative stress. 4) Appropriate antioxidant therapy may reduced this cellular death. 5) Some proteins, probably chaperon, may actively regulate oxidative phosphorylation and autophagy under hypothermia.

Research Products

• [Journal Article] Hydrogen sulfide augments survival signals in warm ischemia and reperfusion of the mouse liver. (2015)
  • Author(s): Shimada S, Fukai M, Wakayama K, Ishikawa T, Kobayashi N, Kimura T, Yamashita K, Kamiyama T, Shimamura T, Taketomi A, Todo S
  • Journal Title: Surg Today Volume: 45 Pages: 892-903
  • Peer Reviewed
• [Journal Article] Intra-arterial infusion of IA-call (R) combined with oral anticancer drugs for recurrent hepatocellular carcinoma after liver resection. (2014)
  • Author(s): Wakayama K, Kamiyama T, Yokoo H, Kakisaka T, Orimo T, Tsuruga Y, Kamachi H, Soyama T, Sakuhara Y, Abo D, Taketomi A
  • Journal Title: Gan To Kagaku Ryoho Volume: 41(12) Pages: 1503-1505
  • Peer Reviewed
• [Journal Article] Hydrogen sulfide augments survival signals in warm ischemia and reperfusion of the mouse liver. (2014)
  • Author(s): Shimada S, Fukai M, Wakayama K, Ishikawa T, Kobayashi N, Kimura T, Yamashita K, Kamiyama T, Shimamura T, Taketomi A, Todo S.
  • Journal Title: Surg Today Volume: epub
  • Peer Reviewed
• [Journal Article] Hand-assisted laparoscopic splenectomy for sclerosing angiomatoid nodular transformation of the spleen complicated by chronic disseminated intravascular coagulation: a case report. (2014)
  • Author(s): Kakisaka T, Kamiyama T, Yokoo H, Orimo T, Wakayama K, Tsuruga Y, Kamachi H, Harada T, Kato F, Yamada Y, Mitsuhashi T, Taketomi A.
  • Journal Title: Asian J Endosc Surg. Volume: 7(3) Issue: 3 Pages: 275-278
  • DOI: 10.1111/ases.12116
  • Peer Reviewed
• [Journal Article] 臓器保存液の現状と今後～単純冷保存は役割を終えたのか？ (2013)
  • Author(s): 深井原, 島田慎吾, 若山顕治, 嶋村剛, 山下健一郎, 藤堂省, 武冨紹信
  • Journal Title: Organ Biology Volume: 20(2) Pages: 176-180
• [Presentation] 低温灌流時の酸化的リン酸化と細胞内シグナルの制御～尿細管上皮を用いた検討 (2015)
  • Author(s): 深井原、島田慎吾、若山顕治、石川隆壽、山下健一郎、嶋村剛、武冨紹信
  • Organizer: 第42回日本臓器保存生物医学会学術集会
  • Place of Presentation: いわて県民情報交流センター（岩手県盛岡市）
  • Year and Date: 2015-11-13
• [Presentation] Time course of autophagy and oxidative phosphorylation during hypothermic oxygenated conditions and subsequent re-warming in human renal tubular cell line (HK2). (2015)
  • Author(s): Fukai M, Ishikawa T, Wakayama K, Shimada S, Fujiyoshi M, Kimura T, Yamashita K, Shimamura T, Taketomi A.
  • Organizer: 17th Congress of the European Society for Organ Transplantation (ESOT)
  • Place of Presentation: SQUARE BRUSSELS MEETING CENTRE (Brussels, Belgium)
  • Year and Date: 2015-09-14
  • Int'l Joint Research
• [Presentation] Signal transduction during hypothermic oxygenated conditions in human renal tubular cells. (2015)
  • Author(s): Fukai M, Ishikawa T, Wakayama K, Shimada S, Kimura T, Yamashita K, Shimamura T, Taketomi A.
  • Organizer: 14th Congress of the Asian Society of Transplantation (CAST)
  • Place of Presentation: Suntec Singapore Convention&Exhibition Centre (Suntec City,Singapore)
  • Year and Date: 2015-08-24
  • Int'l Joint Research
• [Presentation] 低温酸素化体外灌流における水素ガスの肝保護効果 (2015)
  • Author(s): 石川隆壽、深井原、島田慎吾、若山顕治、後藤了一、木村太一、大澤郁朗、山下健一郎、嶋村剛、武冨紹信.
  • Organizer: 第115回日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場（愛知県名古屋市）
  • Year and Date: 2015-04-17
• [Presentation] ヒト肝細胞リソース樹立のための組織片処理法と培養条件の検討 (2015)
  • Author(s): 深井原, 石川隆壽, 島田慎吾, 若山顕治, 柿坂達彦, 敦賀陽介, 折茂達也, 横尾英樹, 蒲池浩文, 神山俊哉, 山下健一郎, 嶋村 剛, 武冨紹信
  • Organizer: 第27回 代用臓器・再生医学研究会総会
  • Place of Presentation: 北海道大学医学部学友会館フラテ (札幌市)
  • Year and Date: 2015-02-28
• [Presentation] 低温下でのエネルギー代謝とApoptosis, Autophagy (2014)
  • Author(s): 深井原，石川隆壽，島田慎吾，若山顕治，後藤了一、山下健一郎，嶋村剛，武冨紹信
  • Organizer: 第41回 日本臓器保存生物医学会学術集会
  • Place of Presentation: 千里ライフサイエンスセンター (吹田市)
  • Year and Date: 2014-11-28
• [Presentation] より良い灌流保存のためのより良い単純冷保存法～重水と水素ガスの新たな可能性 (2014)
  • Author(s): 深井原，石川隆壽，島田慎吾，若山顕治 山下健一郎，嶋村剛，神山俊哉，藤堂省，武冨紹信
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2014-04-03
• [Presentation] ヘリウムガスによる肝冷保存再灌流障害軽減の試み (2014)
  • Author(s): 石川隆壽，深井原，島田慎吾，若山顕治 山下健一郎，嶋村剛，神山俊哉，藤堂省，武冨紹信
  • Organizer: 第114回 日本外科学会定期学術集会
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2014-04-03
• [Presentation] Heavy Water and Hydrogen Gas Confer Protection Against Hepatic Cold Ischemia and Reperfusion Injury in Isolated Perfused Rat Liver (2013)
  • Author(s): Shimada S, Fukai M, Wakayama K, Yamashita K, Taniguchi M, Suzuki T, Shimamura T, Kamiyama T, Furukawa F, Todo S, Taketomi A.
  • Organizer: American Transplant Congress 2013
  • Place of Presentation: Washington State Convention & Trade Center （Seattle, USA）
• [Presentation] Heavy Water and Hydrogen Gas Ameliorates Cold Preservation and Reperfusion Injury in Rat Liver (2013)
  • Author(s): Shimada S, Fukai M, Wakayama K, Yamashita K, Taniguchi M, Suzuki T, Shimamura T, Kamiyama T, Furukawa F, Todo S, Taketomi A.
  • Organizer: Congress of European Society for Organ Transplantation 2013
  • Place of Presentation: Austria Center Vienna（Vienna, Austria）
• [Presentation] より良い臓器灌流のための臓器保存法と再灌流時治療：重水、水素の可能性
  • Author(s): 深井原、島田慎吾、若山顕治、石川隆壽、山下健一郎、嶋村剛、武冨紹信
  • Organizer: 第４０回 日本臓器保存生物医学会学術集会
  • Place of Presentation: 東京医科大学病院臨床講堂（東京都）
• [Presentation] 低温下でエネルギー産生を賦活化させる新しい方法～有効性と普遍性
  • Author(s): 深井原, 若山顕治, 山下健一郎, 廣方玄太郎, 谷口雅彦, 古川博之, 島田慎吾, 小倉正臣, 鈴木友己, 嶋村剛, 尾崎倫孝, 神山俊哉, 藤堂省
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場（福岡市）
• [Presentation] ラット冷保存肝における再灌流時水素ガス投与の効果
  • Author(s): 島田慎吾、深井原, 山下健一郎, 鈴木友己, 嶋村剛, 尾崎倫孝, 神山俊哉、藤堂省
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場（福岡市）