Growth suppression of human breast cancer, based on the clock gene function

• Project/Area Number: 25861148
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Hirosaki University
• Principal Investigator: Morohashi Satoko 弘前大学, 医学(系)研究科(研究院), 助教 (90569592)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 乳癌 / 時計遺伝子 / 治療 / 分子標的治療薬

Outline of Final Research Achievements

The purpase of the staging is growth suppression of human breast cancer, based on the clock gene function.
The expression of c-Myc was upregulated by exposure to hypoxia and by the overexpression of DEC2. In conclusion, DEC2 participates in hypoxia-induced cell proliferation by functioning as a target gene of the PI3K/Akt signaling pathway and regulating the expression of c-Myc in human breast cancer cell line MCF-7. We also demonstrated that DEC1 overexpression promoted cleaved PARP expression, whereas DEC2 overexpression had no effects on the amount of cleaved PARP in TE 10 cells. These results suggested that DEC1 has pro-apoptotic effects on human esophageal cancer TE 10 cells of well-differentiated type.

Research Products

• [Journal Article] Ex vivo application of endocytoscopy for determining the longitudinal extent of bile duct cancer: a pathological analysis of 28 resected cases. (2015)
  • Author(s): Yoshikawa T, Hakamada K, Ogasawara H, Kimura N, Kudo D, Sakamoto Y, Ishido K, Toyoki Y, Morohashi S, Kijima H.
  • Journal Title: J Hepatobiliary Pancreat Sci Volume: 22 Pages: 58-67
  • Peer Reviewed / Open Access
• [Journal Article] Fatty acid synthase-positive hepatocytes and subsequent steatosis in rat livers by irinotecan (2015)
  • Author(s): Sawano T, Shimizu T, Yamada T, Nanashima N, Miura T, Morohashi S, Kudo D, Hui F-M, Kijima H, Hakamada K, Tsuchida S
  • Journal Title: Oncology Reports Volume: 33 Pages: 2151-2160
  • DOI: 10.3892/or.2015.3814
  • Peer Reviewed / Open Access
• [Journal Article] <b>Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer </b><b>cells </b> (2015)
  • Author(s): Seino H
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 89-96
  • DOI: 10.2220/biomedres.36.89
  • NAID: 130005065284
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] 診断に苦慮した乳輪部に発生した巨大尖圭コンジローマの1例 (2015)
  • Author(s): 諸橋聡子, 吉澤 忠司, 平井 秀明, 羽賀 敏博, 清野 浩子, 呉 雲燕, 泉 美貴, 鬼島 宏
  • Journal Title: 診断病理 Volume: 32 Pages: 159-163
  • Peer Reviewed / Open Access
• [Journal Article] Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2. (2015)
  • Author(s): Wu Y, Sato H, Suzuki T, Yoshizawa T, Morohashi S, Seino H, Kawamoto T, Fujimoto K, Kato Y, Kijima H
  • Journal Title: Int J Mol Med Volume: 35 Pages: 815-820
  • Peer Reviewed / Open Access
• [Journal Article] Sentinel lymph node biopsy in male breast cancer - a case report (2014)
  • Author(s): Nishi T, Nishimura A, Morohashi S, Hakamada K.
  • Journal Title: Gan To Kagaku Ryoho. Volume: 41 Pages: 1972-1974
  • Peer Reviewed
• [Journal Article] The case of a long-surviving patient with breast cancer and brain metastases treated using multidisciplinary therapy (2014)
  • Author(s): Nishimura A, Nishi T, Morohashi S, Okano K, Hakamada K.
  • Journal Title: Gan To Kagaku Ryoho. Volume: 41 Pages: 1897-1899
  • Peer Reviewed
• [Journal Article] Pathological characteristics of early to advanced gallbladder carcinoma and extrahepatic cholangiocarcinoma. (2014)
  • Author(s): Kijima H, Wu Y, Yosizawa T, Suzuki T, Tsugeno Y, Haga T, Seino H, Morohashi S, Hakamada K.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21 Issue: 7 Pages: 453-8
  • DOI: 10.1002/jhbp.72
  • Peer Reviewed / Open Access
• [Journal Article] Epithelial cell adhesion molecule (EpCAM) overexpression is correlated with malignant potentials of intraductal papillary mucinous neoplasms (IPMNs) of the pancreas. (2013)
  • Author(s): Yonaiyama S, Toyoki Y, Morohashi S, Sakuraba S, Yoshizawa T, Suzuki T, Wu Y, Kijima H, Hakamada K.
  • Journal Title: Biomed Res. Volume: 34 Pages: 87-95
  • NAID: 130004470840
  • Peer Reviewed
• [Journal Article] 原発性硬化性胆管炎に高分化型胆管癌および潰瘍性大腸炎を併発し長期経過した1剖検例 (2013)
  • Author(s): 布木 誠之, 諸橋 聡子, 清野 浩子, 呉 雲燕, 吉澤 忠司, 鬼島 宏
  • Journal Title: 診断病理 Volume: 30 Pages: 234-239
  • Peer Reviewed
• [Journal Article] Endo-Cytoscopyによる胆管癌進展度診断法の開発 (2013)
  • Author(s): 吉川 徹, 袴田 健一, 小笠原 紘志, 木村 憲央, 工藤 大輔, 石戸 圭之輔, 豊木 嘉一, 諸橋 聡子, 鬼島 宏
  • Journal Title: 胆膵の病態生理 Volume: 29 Pages: 23-29
  • Peer Reviewed
• [Journal Article] 両側副腎褐色細胞腫摘除術26年後に局所再発したvon Hippel-Lindau病随伴褐色細胞腫の1例 (2013)
  • Author(s): 藤田 尚紀, 三上 穣太郎, 村澤 洋美, 岡本 亜希子, 今井 篤, 畠山 真吾, 石村 大史, 米山 高弘, 古家 琢也, 神村 典孝, 大山 力, 諸橋 聡子, 鬼島 宏
  • Journal Title: 泌尿器科紀要 Volume: 7 Pages: 427-430
  • NAID: 120005308136
  • Peer Reviewed
• [Presentation] 乳腺穿刺吸引細胞診鑑別困難例の細胞像と組織像の対比検討 (2015)
  • Author(s): 諸橋聡子 刀稱亀代志 吉澤忠司 平井秀明 羽賀敏博 黒瀬 顕 鬼島 宏
  • Organizer: 第54回日本臨床細胞学会秋期大会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋市）
  • Year and Date: 2015-11-21
• [Presentation] 乳腺穿刺吸引細胞診の精度管理 鑑別困難例から学ぶこと (2015)
  • Author(s): 諸橋聡子 吉澤忠司 平井秀明 羽賀敏博 呉雲燕 鬼島宏
  • Organizer: 第52回日本臨床細胞学会東北支部連合会学術集会
  • Place of Presentation: 山形テルサ（山形県・山形市）
  • Year and Date: 2015-07-11
• [Presentation] A huge condyloma acuminatum that occurred in the areola part (2015)
  • Author(s): 諸橋聡子 平井秀明 羽賀敏博 吉澤忠司 清野浩子 呉雲燕 泉 美貴 鬼島宏
  • Organizer: 第104回 日本病理学会総会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋市）
  • Year and Date: 2015-04-30
• [Presentation] 早期胃癌におけるリンパ管・血管の局在解析 (2014)
  • Author(s): 佐々木花恵、諸橋聡子、鳥羽崇仁、吉澤忠司、羽賀敏博、平井秀明、清野浩子、呉雲燕、鬼島宏
  • Organizer: 第103回日本病理学会誌
  • Place of Presentation: 広島国際会議場
  • Year and Date: 2014-04-24 – 2014-04-26
• [Presentation] センチネルリンパ節の病理診断の工夫 (2013)
  • Author(s): 諸橋 聡子, 菅原 和子, 清野 浩子, 呉 雲燕, 西村 顕正, 西 隆, 鬼島 宏, 袴田 健一
  • Organizer: 日本乳癌学会
  • Place of Presentation: アクトシティ浜松市
• [Presentation] BHLH転写因子DEC2はBxPC-3細胞においてTGF-β誘導性腫瘍の進行を抑制する(BHLH transcription factor DEC2 inhibits TGF-β-induced tumor progression in BxPC-3 cells)(英語) (2013)
  • Author(s): 呉 雲燕, 清野 浩子, 鈴木 貴弘, 諸橋 聡子, 吉澤 忠司, 加藤 幸夫, 鬼島 宏
  • Organizer: 日本病理学会
  • Place of Presentation: ロイトン札幌