Rat islet long-term culture in serum-free medium containing silk protein sericin and albumin
| Project/Area Number |
25861151
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General surgery
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| Research Institution | University of Fukui |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | sericin / 膵島移植 / 膵島培養 / ラット / 無血清培地 / アルブミン |
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| Outline of Final Research Achievements |
We reported islets can be short-term cultured for 14 days using serum-free medium containing sericin instead of FBS.The study evaluated the use of the medium containing sericin and albumin for islet long-term culture. Twenty rat islets were cultured in medium containing ①FBS,②sericin,③bovine serum albumin(BSA),④sericin+BSA,⑤FBS+sericin. The cultured islets in ②and③ were disappered for either 35 and 77days. The survival rates for 120 days of ①,④and⑤ were either 24,24,and 13%. Insulin secretions of the ①and④ were measured by static incubation on the day120, and no significant differences in stimulated insulin secretion were noted between the groups. In vivo function of cultured islets of ①and④ were tested by syngeneic transplantation for diabetes rats, and both recipients were maintained normal glycemic control for 28 days after transplantation. Histologically, islets after transplantation with ④ was displayed well-preserved structure and strong insulin staining.
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Report
(3 results)
Research Products
(2 results)
