Prediction method for developing pouchitis in patients with ulcerative colitis by using genetic information on ileal mucosal epithelial cells

• Project/Area Number: 25861181
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: YOSHIKI Okita 三重大学, 医学部附属病院, 助教 (20378342)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 回腸嚢炎 / 潰瘍性大腸炎 / pouchitis / micro RNA

Outline of Final Research Achievements

FKBP5 mRNA levels in ileal mucosa at the time of colectomy are significantly associated with pouchitis and may be a predictive factor for developing pouchitis. IL-1βmRNA levels are also significantly associated with pouchitis and it is correlated to extent of disease and disease severity. IL-1β may be a predictive factor for developing pouchitis. Comprehensive miRNA expression patterns related to pouchitis was analyzed by using ileal mucosa of postoperative ulcerative colitis patients. Eleven miRNA was detected and confirmed in other samples.

Research Products

• [Journal Article] FK506-Binding Protein 5 mRNA Levels in Ileal Mucosa Are Associated with Pouchitis in Patients with Ulcerative Colitis. (2015)
  • Author(s): Araki T, Kawamura M, Tanaka K, Okita Y, Fujikawa H, Uchida K, Toiyama Y, Inoue Y, Mohri Y, Kusunoki M
  • Journal Title: Dig Dis Sci. Volume: 該当なし Issue: 6 Pages: 1617-1623
  • DOI: 10.1007/s10620-015-3528-9
  • Peer Reviewed
• [Journal Article] Colonic histological criteria predict development of pouchitis after ileal pouch–anal anastomosis for patients with ulcerative colitis (2015)
  • Author(s): Araki T, Hashimoto k, Okita Y, Fujikawa H, Kondo S, Kawamura M, Kobayashi M, Ohi M, Tanaka K, Toiyama Y, Inoue Y, Uchida K, Mohri Y, Kusunoki M
  • Journal Title: Hepatogastroenterology Volume: 該当なし
  • Peer Reviewed
• [Journal Article] Characteristics of extremely early-onset pouchitis after proctocolectomy with ileal pouch-anal anastomosis. (2013)
  • Author(s): Okita Y, Araki T, Tanaka K, Shimura T, Kawamura M, Fujikawa H, Inoue M, Inoue Y, Uchida K, Mohri Y,Kusunoki M
  • Journal Title: J Gastrointest Surg Volume: 17 Issue: 3 Pages: 533-539
  • DOI: 10.1007/s11605-012-2120-2
  • Peer Reviewed
• [Journal Article] Predictive factors for development of chronic pouchitis after ileal pouch-anal anastomosis in ulcerative colitis. (2013)
  • Author(s): Okita Y, Araki T, Tanaka K, Hashimoto K, Kondo S, Kawamura M, Koike Y, Otake K, Fujikawa H, Inoue M, Ohi M, Inoue Y, Uchida K, Mohri Y, Kusunoki M.
  • Journal Title: Digestion. Volume: 88 Issue: 2 Pages: 101-109
  • DOI: 10.1159/000353151
  • Peer Reviewed
• [Presentation] 潰瘍性大腸炎術後回腸嚢炎と回腸嚢粘膜におけるinflammatory cytokines発現との関連性 (2015)
  • Author(s): 大北喜基，荒木俊光，川村幹雄，長野由佳，近藤哲，三枝晋，問山裕二， 田中光司，井上靖浩，内田恵一，毛利靖彦，楠 正人
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-04-16 – 2015-04-18
• [Presentation] 潰瘍性大腸炎術後回腸嚢炎発症と大腸全摘時回腸粘膜中inflammatory cytokinesの発現との関連性 (2015)
  • Author(s): 川村幹雄，荒木俊光，大北喜基，長野由佳，近藤哲，三枝晋，問山裕二，田中光司，井上靖浩，内田恵一，毛利靖彦，楠 正人
  • Organizer: 第115回 日本外科学会定期学術集会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2015-04-16 – 2015-04-18
• [Presentation] The relationship between onset days and clinical characteristics of pouchitis after ileal pouch-anal anastomosis in ulcerative colitis (2015)
  • Author(s): Yoshiki Okita, Toshimitsu Araki, Yuka Nagano, Satoru Kondo, Mikio Kawamura, Mikihiro Inoue, Yuji Toiyama, Masaki Ohi, Koji Tanaka, Yasuhiro Inoue, Keiichi Uchida, Yasuhiko Mohri, Masato Kusunoki
  • Organizer: 10th Congress of ECCO
  • Place of Presentation: CCIB Barcelona, Spain
  • Year and Date: 2015-02-18 – 2015-02-21
• [Presentation] Predictive factors for development of chronic pouchitis after ileal pouch-anal anastomosis in ulcerative colitis (2014)
  • Author(s): Yoshiki Okita, Toshimitsu Araki, Masato Kusunoki
  • Organizer: JDDW 2014
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2014-10-23 – 2014-10-26
• [Presentation] 潰瘍性大腸炎手術症例における回腸粘膜内forkhead box protein遺伝子発現の意義 (2014)
  • Author(s): 大北喜基，荒木俊光，藤川裕之，川村幹雄，北嶋貴仁，近藤哲，志村匡信，井上幹大，問山裕二，小林美奈子，大井正貴，田中光司，井上靖浩，内田恵一，毛利靖彦，楠正人
  • Organizer: 第100回日本消化器病学会総会
  • Place of Presentation: 東京国際フォーラム
  • Year and Date: 2014-04-23 – 2014-04-26
• [Presentation] Predictive factors for development of chronic pouchitis after ileal pouch-anal anastomosis in ulcerative colitis (2013)
  • Author(s): Yoshiki Okita, Toshimitsu Araki, Koji Tanaka, Hiroyuki Fujikawa, Mikihiro Inoue, Masaki Ohi,Yasuhiro Inoue, Keiichi Uchida, Yasuhiko Mohri, Masato Kusunoki
  • Organizer: American College of Surgeons' 99th Clinical Congress 2013
  • Place of Presentation: Washington DC