Analysis of induced cancer stem-like sphere cells from de-differentiated human hepatocellular carcinoma cell lines to examine the molecular targets for the cancer therapy
Project/Area Number |
25861192
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Yamaguchi University |
Principal Investigator |
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Project Status |
Completed (Fiscal Year 2015)
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Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | 癌 / がん幹細胞 |
Outline of Final Research Achievements |
Cancer stem cells (CSCs) are thought to play important roles in therapy-resistance. In this study, we induced cancer stem-like cells from hepatocellular carcinoma (HCC) cell lines using a unique medium, and examined their potential for resistance to anti-cancer drugs. Poorly differentiated HCC derived cell lines efficiently formed spheres of cells, but well-differentiated HCC-derived cells did not. Sphere cells showed increased stemness marker levels. We observed more CD44 variant-positive cells in Sphere cells than in parental cells. The cell viability of Sphere cells was significantly higher than that of parental cells in the presence of several anti-cancer drugs. Sphere cells showed higher ABCG2 and HIF1A mRNA expression and lower ROS production compared to parental cells. Our novel method successfully induced cancer stem-like cells, which possessed chemoresistance that was related to the cell cycle, drug efflux, and ROS.
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Report
(4 results)
Research Products
(43 results)
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[Journal Article] Involvement of angiotensin II type 2 receptor (AT2R) signaling in human pancreatic ductal adenocarcinoma (PDAC): a novel AT2R agonist effectively attenuates growth of PDAC grafts in mice.2015
Author(s)
Ishiguro S, Yoshimura K, Tsunedomi R, Oka M, Takao S, Inui M, Kawabata A, Wall T, Magafa V, Cordopatis P, Tzakos AG, Tamura M.
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Journal Title
Cancer Biol Ther.
Volume: 16
Issue: 2
Pages: 307-316
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Relationship between Polymorphisms and the Efficacy of Cetuximab2015
Author(s)
Yuka Inoue, Shoichi Hazama, Ryouichi Tsunedomi, Sigeru Takeda, Tomio Ueno, Sigeru Yamamoto, Shigefumi Yoshino, Junichi Sakamoto, Hideyuki Mishima, Masaaki Oka, and Hiroaki Nagano
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Journal Title
Gan To Kagaku Ryoho
Volume: 42
Pages: 1310-1312
Related Report
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[Journal Article] Preoperative serum methylation signature as prognostic tool after curative hepatectomy in patients with hepatocellular carcinoma2015
Author(s)
Shinsuke Kanekiyo, Norio Iizuka, Ryouichi Tsunedomi, Yukio Tokumitsu, Noriaki Hashimoto, Yoshihiro Tokuhisa, Yoshinari Maeda, Michihisa Iida, Kazuhiko Sakamoto, Takao Tamesa, Yusuke Fujita, Shigefumi Yoshino, Shoichi Hazama, Yoshihiko Hamamoto, and Masaaki Oka
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Journal Title
Anticancer Res
Volume: 35
Pages: 997-1007
Related Report
Peer Reviewed
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[Journal Article] A phase ΙI study of five peptides combination with oxaliplatin-based chemotherapy as a first-line therapy for advanced colorectal cancer (FXV study).2014
Author(s)
Hazama S, Nakamura Y, Tanaka H, Hirakawa K, Tahara K, Shimizu R, Ozasa H, Etoh R, Sugiura F, Okuno K, Furuya T, Nishimura T, Sakata K, Yoshimatsu K, Takenouchi H, Tsunedomi R, Inoue Y, Kanekiyo S, Shindo Y, Suzuki N, Yoshino S, Shinozaki H, Kamiya A, Furukawa H, Yamanaka T, Fujita T, Kawakami Y, Oka M.
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Journal Title
J Transl Med.
Volume: 12
Issue: 1
Pages: 108-108
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Predictive Biomarkers for the Outcome of Vaccination of Five Therapeutic Epitope Peptides for Colorectal Cancer2014
Author(s)
Shoichi Hazama, Hiroko Takenouchi, Ryouichi Tsunedomi, Michihisa Iida, Nobuaki Suzuki, Norio Iizuka, Yuka Inoue, Kazuhiko Sakamoto, Mitsuhiro Nakao, Yoshitaro Shindo, Shinsuke Kanekiyo, Yukio Tokumitsu, Kiyoshi Yoshimura, Noriko Maeda, Kazunari Maeda, Yoshinari Maeda, Hiroto Matsui, Shigefumi Yoshino, et al.
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Journal Title
Anticancer Research
Volume: 34
Pages: 4201-4205
Related Report
Peer Reviewed
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[Journal Article] A stem cell medium containing neural stimulating factor induces a pancreatic cancer stem-like cell-enriched population.2014
Author(s)
Watanabe Y, Yoshimura K, Yoshikawa K, Tsunedomi R, Shindo Y, Matsukuma S, Maeda N, Kanekiyo S, Suzuki N, Kuramasu A, Sonoda K, Tamada K, Kobayashi S, Saya H, Hazama S and Oka M
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Journal Title
Int J Oncol
Volume: 45
Issue: 5
Pages: 1857-1866
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Differences in UGT1A1, UGT1A7, and UGT1A9 Polymorphisms between Uzbek and Japanese Populations2014
Author(s)
Hiromichi Maeda, Shoichi Hazama, Abdiev Shavkat, Ken Okamoto, Koji Oba, Junichi Sakamoto, Kenichi Takahashi, Masaki Oka, Daisuke Nakamura, Ryouichi Tsunedomi, Naoko Okayama, Hideyuki Mishima, Michiya Kobayashi
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Journal Title
Molecular diagnosis & therapy
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Expression of B7-H3, a Potential Factor of Tumor Immune Evasion in Combination with the Number of Regulatory T Cells, Affects Against Recurrence-Free Survival in Breast Cancer Patients2014
Author(s)
Noriko Maeda, Kiyoshi Yoshimura, Sigeru Yamamoto, Atsuo Kuramasu, Moeko Inoue, Nobuaki Suzuki, Yusaku Watanabe, Yoshinari Maeda, Ryoji Kamei, Ryouichi Tsunedomi, Yoshitaro Shindo, Makoto Inui, Koji Tamada, Shigefumi Yoshino, Shoichi Hazama, Masaaki Oka
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Journal Title
Annals of Surgical Oncology
Volume: in press
Related Report
Peer Reviewed
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[Presentation] OVEREXPRESSION OF MIRNA221 NAD 222 IN CANCER STROMA IS ASSOCIATED WITH MALIGNANT POTENTIALS IN COLORECTAL CANCER2015
Author(s)
Michihisa Iida, Shoichi Hazama, Ryouichi Tsunedomi, Takao Tamesa, Shigeru Takeda, Tomio Ueno, Shigeru Yamamoto, Shigefumi Yoshino, Yutaka Kawakami, and Hiroaki Nagano
Organizer
23nd United European Gastroenterology Week
Place of Presentation
Barcelona, SPAIN
Year and Date
2015-10-26
Related Report
Int'l Joint Research
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[Presentation] miR expression of the tumor might be a predictive biomarker for the efficacy of peptide vaccine for colorectal cancer2015
Author(s)
Shoichi Hazama, Yuka Inoue, Shinsuke Kanekiyo, Yoshitaro Shindo, Nobuaki Suzuki, Takao Tamesa, Ryouichi Tsunedomi, Shigeru Takeda, Tomio Ueno, Shigefumi Yoshino, Yusuke Nakamura, Yutaka Kawakami, and Hiroaki Nagano
Organizer
第74回日本癌学会学術総会
Place of Presentation
名古屋国際会議場 (愛知県)
Year and Date
2015-10-10
Related Report
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[Presentation] Calreticulin is highly expressed in pancreatic cancer stem like cells2015
Author(s)
Satoshi Matsukuma, Kiyoshi Yoshimura, Yusaku Watanabe, Ryoichi Tsunedomi, Moeko Inoue, Tomoko Kondo-Furuya, Atsunori Oga, Tomio Ueno, Shigefumi Yoshino, Shoichi Hazama, Hiroshi Ito, and Hiroaki Nagano
Organizer
第74回日本癌学会学術総会
Place of Presentation
名古屋国際会議場 (愛知県)
Year and Date
2015-10-08
Related Report
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[Presentation] Biomarkers for immunotherapy: Results from the analysis of a HLA-A-status double-blind, biologically-randomized phase II study of five therapeutic epitope-peptides with oxaliplatin-based chemotherapy as first-line therapy for advanced colorectal cancer (FXV study)2014
Author(s)
Shoichi Hazama, Yusuke Nakamura, Hiroaki Tanaka, Fumiaki Sugiura, Kiyotaka Okuno, Kazuhiko Yoshimatsu, Hiroko Takenouchi, Ryouichi Tsunedomi, Yuka Inoue, Shinsuke Kanekiyo, Yoshitaro Shindo, Kiyoshi Yoshimura, Shigefumi Yoshino, Tomonobu Fujita, Yutaka Kawakami, and Masaaki Oka
Organizer
第18回日本がん免疫学会総会
Place of Presentation
ひめぎんホール (愛媛県)
Year and Date
2014-07-31
Related Report
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[Presentation] Expressions of ULBP1 and NKG2D are related to their clinicopathological outcome in patients with gastric cancer2013
Author(s)
Kiyoshi Yoshimura, Ryoji Kamei, Nobuaki Suzuki, Ryouichi Tsunedomi, Noriko Maeda, Yusaku Watanabe, Yoshinari Maeda, Shigeru Takeda, Tomio Ueno, Shigeru Yamamoto, Shigefumi Yoshino, Shoichi Hazama, Masaaki Oka
Organizer
第72回日本癌学会学術総会
Place of Presentation
パシフィコ横浜 (神奈川県)
Related Report
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