Development of novel therapies that focus on specific stroma cell induced peritoneal dissemination of pancreatic cancer

• Project/Area Number: 25861198
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: MORIYAMA Taiki 九州大学, 大学病院, 助教 (70586859)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 膵癌 / 筋線維芽細胞 / 腹膜播種 / 癌間質相互作用 / 腹膜中皮細胞 / 線維芽細胞

Outline of Final Research Achievements

This study was designed to assess the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer. Human peritoneal myofibroblasts (hPMFs) were established from disseminated sites of pancreatic cancer and their interactions with the SUIT-2 and CAPAN-1 human pancreatic cancer cell lines were analyzed in vitro and in vivo. In vitro, hPMFs significantly promoted the migration and invasion of pancreatic cancer cells (P<0.05), while the cancer cells significantly promoted the migration and invasion of hPMFs (P<0.05). In vivo, the number of perito-neally disseminated nodules was significantly greater in mice implanted with cancer cells plus hPMFs compared to mice implanted with cancer cells alone.
HPMFs promote the peritoneal dissemination of pancreatic cancer. The cancer-stromal cell interaction in the peritoneal cavity may be a new therapeutic target to prevent the dissemination of pancreatic cancer.

Research Products

• [Journal Article] Peritoneal myofibroblasts at metastatic foci promote dissemination of pancreatic cancer. (2014)
  • Author(s): Akagawa S, Ohuchida K, Torata N, Hattori M, Eguchi D, Fujiwara K, Kozono S, Cui L, Ikenaga N, Ohtsuka T, Oishima S, Mizumoto K, Oda Y, Tanaka M
  • Journal Title: Int J Oncol Volume: 45 Issue: 1 Pages: 113-120
  • DOI: 10.3892/ijo.2014.2391
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] 腹膜線維芽細胞の作用に着目した膵癌腹膜藩種メカニズムの解明
  • Author(s): 赤川進
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡市
• [Presentation] Comparisons of Characteristics of Peritoneal Fibroblasts in Dissemination with those of Mesothelial Cells and Pancreatic Stellate
  • Author(s): Akagawa S
  • Organizer: American Pancreatic Association, 44th Annual Meeting
  • Place of Presentation: Miami, USA