Pancreatic stellate cells specific HSP47 genetic control promote pancreatic regeneration

• Project/Area Number: 25861207
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Sapporo Medical University
• Principal Investigator: OTA Shigenori 札幌医科大学, 医学部, 研究員 (90457705)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 膵再生 / 膵星細胞 / 再生医療 / HSP47 / 薬物輸送システム

Outline of Final Research Achievements

Pancreatic acinar cells(Pacs) and Islet cells(Ics) vigorously proliferated after 90% pancreatectomy. In addition, Pacs proliferation was facilitated by co-culture with pancreatic stellate cells(Pscs). HSP 47 activation in Pscs plays an important role in the proliferation of Pacs, which was totally blocked by HSP47-specific siRNA delivery in vivo and in vitro. Therefore, HSP47 expression in Pscs would be necessary to promote pancreatic regeneration.

Research Products

• [Journal Article] Activated hepatic stellate cells are dependent on self-collagen, cleaved by membrane type 1 matrix metalloproteinase for their growth. (2014)
  • Author(s): Birukawa NK, Murase K, Sato Y, Kosaka A, Yoneda A, Nishita H, Fujita R, Nishimura M, Ninomiya T, Kajiwara K, Miyazaki M, Nakashima Y, Ota S, Murakami Y, Tanaka Y, Minomi K, Tamura Y, Niitsu Y
  • Journal Title: the journal of biological chemistry Volume: 289(29) Pages: 20209-20221
  • Peer Reviewed
• [Journal Article] What's new in surgery frontier 第78回 肝炎・肝硬変と星細胞 (2013)
  • Author(s): 太田 盛道
  • Journal Title: Surgery Frontier Volume: 20巻3号 Pages: 316-318
• [Presentation] Vitamin A-Binding Liposome Containing Heat Shock Protein 47 siRNA Regulates Pancreatic Regeneration (2014)
  • Author(s): Shigenori Ota
  • Organizer: 第45回日本膵臓学会・米国膵臓学会合同記念大会
  • Place of Presentation: ハワイ島 (アメリカ)
  • Year and Date: 2014-11-05 – 2014-11-08
• [Presentation] 星細胞特異的DDS を用いた線維化溶解療法の臨床応用に向けた基礎研究 (2014)
  • Author(s): 太田 盛道
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
• [Presentation] HSP47遺伝子制御による膵星細胞特異的制御と膵再生の検証 (2014)
  • Author(s): 太田 盛道
  • Organizer: 第69回日本消化器外科学会総会
  • Place of Presentation: 福島