Functional analysis of pigment epithelium-derived factor (PEDF) in atherosclerotic lesions

• Project/Area Number: 25861231
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Kyushu University
• Principal Investigator: KYURAGI Ryoichi 九州大学, 医学(系)研究科(研究院), 研究員 (20631592)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 血管新生抑制 / 色素上皮由来因子

Outline of Final Research Achievements

There were several reports of the pigment epithelium-derived factor (PEDF) has a neuroprotective effect and strong anti-angiogenic effect so far. We therefore tried to product the tissue-specific overexpression of PEDF transgenic mice. We attempted to analysis interaction between PEDF and various vascular lesions through using vascular animal model. However, the transgenic mouse constructs have been able to prepare, which was not introduced into the ES cells. Gene transfer into fertilized eggs were also tried but did not reach PEDF transgenic mouse production.