Enhanced Sternal Healing via Effect of Controlled Release and Spatial Retention of Platelet-Rich Plasma Impregnated in Biodegradable Gelatin Hydrogel

• Project/Area Number: 25861234
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cardiovascular surgery
• Research Institution: Nippon Medical School
• Principal Investigator: Jiro Kurita 日本医科大学, 医学部, 助教 (20421183)
• Research Collaborator: SHIBATA Masafumi
• Project Period (FY): 2013-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 再生医療 / 血管新生 / 多血小板血漿 / 心臓手術 / 創傷治癒 / 心臓血管外科 / 胸部外科 / 新生血管

Outline of Final Research Achievements

Purpose: The aim of this study was to evaluate the effectiveness of the controlled release of PRP. Methods: After median sternotomy and bilateral internal thoracic artery removal, 16 rabbits were randomized into 4 groups: control (ctrl), 30mg of gelatin-hydrogel incorporating 300μl of PBS (gel), 300μl of the solution form of PRP (PRP), 30mg of gelatin-hydrogel incorporating 300μl of PRP (PRP+gel). Results: This study demonstrated that a controlled release of PRP increases the bone mineral density of sponge bone (382.7±40.1mg/cm3) compared with Ctrl, gel, and PRP (265.4±18.9, 325.6±41.4, 285.4±31.5mg/cm3, respectively p<0.01). Histologic analysis of PRP+gel group revealed accumulation of fibrous tissue into the separated sternal space. Apparent sternal dehiscence was observed in the Ctrl, gel, PRP as assessed radio graphically. Conclusion: Controlled release PRP system is markedly effective in encouraging sternal healing on early phase.

Research Products

• [Presentation] DDS 徐放化PRP による胸骨正中切開後の胸骨癒合促進における新治療法の開発 (2017)
  • Author(s): 芝田匡史 栗田二郎 宮城泰雄 新田隆
  • Organizer: 第117回日本外科学会定期学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2017-04-27
• [Presentation] Enhanced Sternal Healing via Effect of Controlled Release and Spatial Retention of Platelet-Rich Plasma Impregnated in Biodegradable Gelatin Hydrogel (2016)
  • Author(s): Masafumi Shibata; Jiro Kurita; Yasuo Miyagi; Takashi Nitta
  • Organizer: AHA Scientific Sessions 2016
  • Place of Presentation: New Orleans, LA
  • Year and Date: 2016-11-12
  • Int'l Joint Research