| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Abdominal aortic aneurysm (AAA) is characterized by chronic inflammation and destruction of aortic walls with no established pharmacotherapy. In this study, we investigated into the role of IL-6 in AAA pathogenesis using mouse model of AAA that was induced by periaortic application of CaCl2. Systemic administration of the IL-6 receptor blocking antibody (MR16-1) suppressed formation of AAA in mice. Histological findings indicated less destruction of AAA wall with MR16-1 treatment. Immunofluorescence staining and imaging cytometric analysis revealed the activation of Smad2, an effector of TGF-beta signal. Consistently, biochemical studies showed suppression of MMP-2 and enhancement of lysyl oxidase expression. Therefore, IL-6 promotes AAA formation in the mouse model and its suppression has a therapeutic potential.
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