| Project/Area Number |
25861244
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KAWAGUCHI Koji 名古屋大学, 医学部附属病院, 病院講師 (10402611)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 悪性胸膜中皮腫 / CD44 / バリアントアイソフォーム / ヒアルロン酸 / 細胞株樹立 |
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| Outline of Final Research Achievements |
Malignant mesothelioma is one of poor prognostic disease, which is associated with asbestos exposure. In pleural effusion of malignant mesothelioma patients, much amount of hyaluronic acid are released and used for a diagnostic marker. Using malignant mesothelioma cell lines, we analyzed CD44 expression level and variant isoforms status, which is known to hyaluronic acid receptor and surface marker of cancer stem cell. Furthermore, we also investigated causative genes of secreting hyaluronic acid and conducted integrative analysis to detect CD44 target signal in malignant mesothelioma. As the result, some mesothelioma cell lines secreted much amount of hyaluronic acid and showed high expression level of HAS2 as one of the responsibility gene. Moreover, mesothelioma cell lines harboring HAS2 high expression showed high expression level of CD44 variant isoform v2-v7.
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