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Development of a novel system for capturing circulating tumor cells (CTCs) undergoing EMT in lung cancer.

Research Project

Project/Area Number 25861251
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Respiratory surgery
Research InstitutionUniversity of Occupational and Environmental Health, Japan

Principal Investigator

CHIKAISHI Yasuhiro  産業医科大学, 医学部, 助教 (70609221)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords肺癌 / 循環腫瘍細胞 / 上皮間葉移行 / マイクロ流体チップ / 循環腫瘍細胞(CTC) / 上皮間葉転換(EMT) / 上皮接着分子(EpCAM) / CTC-chip
Outline of Final Research Achievements

The “CellSerach” system is widely used to detect circulating tumor cells (CTCs), but tumor cells undergoing epithelial-mesenchymal transition (EMT) may not be captured with the “CellSearch” because an antibody against EpCAM, an antigen expressing on epithelial cells, is used to capture CTCs. Accordingly, we employed a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated, and evaluated the efficiency in capturing CTCs in experimental studies. The “CTC-chip” provided similar efficiency to “CellSeach” in capturing PC-9 cells with EpCAM expression. In capturing MESO-4 cells without EpCAM expression, the “CTC-chip” conjugated with an anti-podoplanin antibody provided a high efficiency (55-70%) whereas MESO-4 cells were not captured with the “CellSearch”.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (5 results)

All 2015 2014 2013

All Presentation (5 results) (of which Int'l Joint Research: 2 results)

  • [Presentation] Improved efficacy in capturing EpCAM-negative tumor cells using a “Universal CTC-chip”2015

    • Author(s)
      Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Takashi Ohnaga, Fumihiro Tanaka
    • Organizer
      AACR Tumor metastasis 2015
    • Place of Presentation
      Austin, USA
    • Year and Date
      2015-11-30
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Capture of EpCAM-negative circulating tumor cells (CTCs) with a “Universal CTC-Chip”2015

    • Author(s)
      Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Tomoko So, Hidetaka Uramoto, Takashi Ohnaga, Fumihiro Tanaka
    • Organizer
      AACR Annual Meeting 2015
    • Place of Presentation
      Philadelphia, USA
    • Year and Date
      2015-04-18
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparison of CellSearch with polymeric microfluidic devices for CTC isolation using EpCAM-negative tumor cell lines of malignant pleural mesothelioma2014

    • Author(s)
      Yasuhiro Chikaishi1), Tomoko So1), Masaru Takenaka1), Soichi Oka1), Ayako Hirai1), Takashi Iwanami1), Kazue Yoneda1), Hidehiko Shimokawa1), Yoshika Nagata1), Hidetaka Uramoto1), Takeshi Ohnaga2), and Fumihiro Tanaka1)
    • Organizer
      AACR meeting
    • Place of Presentation
      San Diego CA(USA)
    • Year and Date
      2014-04-05 – 2014-04-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Development of system to detect circulating tumor cell in malignant pleural mesothelioma2013

    • Author(s)
      Yasuhiro Chikaishi, Tomoko So, Masaru Takenaka, Soichi Oka, Makoto Nakagawa, Hidehiko Shimokawa, Teruo Iwata, Hidetaka Uramoto, Takeshi Hanagiri, Fumihiro Tanaka, Takeshi Ohnaga
    • Organizer
      AACR meeting
    • Place of Presentation
      Washington,USA
    • Related Report
      2013 Research-status Report
  • [Presentation] 循環腫瘍細胞(CTC)の検出方法の開発2013

    • Author(s)
      近石泰弘、宗 知子、米田和恵、浦本秀隆、花桐武志、大永 崇、田中文啓
    • Organizer
      BioJapan2013
    • Place of Presentation
      横浜
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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