Development of a novel system for capturing circulating tumor cells (CTCs) undergoing EMT in lung cancer.

• Project/Area Number: 25861251
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Respiratory surgery
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: CHIKAISHI Yasuhiro 産業医科大学, 医学部, 助教 (70609221)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 肺癌 / 循環腫瘍細胞 / 上皮間葉移行 / マイクロ流体チップ / 循環腫瘍細胞(CTC) / 上皮間葉転換(EMT) / 上皮接着分子(EpCAM) / CTC-chip

Outline of Final Research Achievements

The “CellSerach” system is widely used to detect circulating tumor cells (CTCs), but tumor cells undergoing epithelial-mesenchymal transition (EMT) may not be captured with the “CellSearch” because an antibody against EpCAM, an antigen expressing on epithelial cells, is used to capture CTCs. Accordingly, we employed a novel microfluidic device (“CTC-chip”) in which any antibody to capture CTCs is easily conjugated, and evaluated the efficiency in capturing CTCs in experimental studies. The “CTC-chip” provided similar efficiency to “CellSeach” in capturing PC-9 cells with EpCAM expression. In capturing MESO-4 cells without EpCAM expression, the “CTC-chip” conjugated with an anti-podoplanin antibody provided a high efficiency (55-70%) whereas MESO-4 cells were not captured with the “CellSearch”.

Research Products

• [Presentation] Improved efficacy in capturing EpCAM-negative tumor cells using a “Universal CTC-chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: AACR Tumor metastasis 2015
  • Place of Presentation: Austin, USA
  • Year and Date: 2015-11-30
  • Int'l Joint Research
• [Presentation] Capture of EpCAM-negative circulating tumor cells (CTCs) with a “Universal CTC-Chip” (2015)
  • Author(s): Kazue Yoneda, Yasuhiro Chikaishi, Eri Kawashima, Tomoko So, Hidetaka Uramoto, Takashi Ohnaga, Fumihiro Tanaka
  • Organizer: AACR Annual Meeting 2015
  • Place of Presentation: Philadelphia, USA
  • Year and Date: 2015-04-18
  • Int'l Joint Research
• [Presentation] Comparison of CellSearch with polymeric microfluidic devices for CTC isolation using EpCAM-negative tumor cell lines of malignant pleural mesothelioma (2014)
  • Author(s): Yasuhiro Chikaishi1), Tomoko So1), Masaru Takenaka1), Soichi Oka1), Ayako Hirai1), Takashi Iwanami1), Kazue Yoneda1), Hidehiko Shimokawa1), Yoshika Nagata1), Hidetaka Uramoto1), Takeshi Ohnaga2), and Fumihiro Tanaka1)
  • Organizer: AACR meeting
  • Place of Presentation: San Diego CA(USA)
  • Year and Date: 2014-04-05 – 2014-04-09
• [Presentation] Development of system to detect circulating tumor cell in malignant pleural mesothelioma (2013)
  • Author(s): Yasuhiro Chikaishi, Tomoko So, Masaru Takenaka, Soichi Oka, Makoto Nakagawa, Hidehiko Shimokawa, Teruo Iwata, Hidetaka Uramoto, Takeshi Hanagiri, Fumihiro Tanaka, Takeshi Ohnaga
  • Organizer: AACR meeting
  • Place of Presentation: Ｗashington,USA
• [Presentation] 循環腫瘍細胞（CTC）の検出方法の開発 (2013)
  • Author(s): 近石泰弘、宗 知子、米田和恵、浦本秀隆、花桐武志、大永 崇、田中文啓
  • Organizer: BioJapan2013
  • Place of Presentation: 横浜