| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Outline of Final Research Achievements |
Notch was selected as an important signaling molecule for cancer stem/initiating cells to maintain stemness, induce cell proliferation and regulate apoptosis. Notch signal inhibition by γ-secretase inhibitor may be effective strategy for the treatment of cancer stem/initiating cells. We analyzed 3 patient’s derived GBM stem-like cells with treatment by MRK003, a novel clinically available γ-secretase inhibitor. Notch 1 and 2 were expressed in all cells. MRK003 suppressed Notch signaling in all cells. Akt signaling which is downstream of Notch was strongly inhibited in two species of cells. These cells showed strong effect of MRK in terms of inhibition of proliferation and sphere formation. On the contrary, the cell without alteration of Akt signaling by MRK showed low effect of MRK. Taken together, the effect of MRK003 may depend on the inhibition of Akt pathway.
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