| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Outline of Final Research Achievements |
5-aminolevulinic acid (5-ALA) induced photodynamic diagnosis (PDD) and photodynamic therapy (PDT) are used for patients with glioblastoma multiforme (GBM). The goal of this study was to investigate the effects of 5-ALA-induced PDD and PDT in GS cells in vitro.Three GS cell lines (G144, G179 and Y10) were obtained as primary cultures from GBM specimens. Four hours after addition of 5-ALA, PDD was evaluated based on detection of PpIX fluorescence using flow cytometry. The effect of PDT on the cells was assessed after irradiation at 20 J/cm2 using an argon-pumped dye laser.PpIX was observed in more than 70% of GS cells from just after washing out of 5-ALA until 6 hours later. The GS cell death rate was 89% after PDT.5-ALA-induced PDD and PDT are very effective for GS cells in vitro. These photodynamic techniques may be useful for diagnosis and therapy associated with GS cells.
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