Curetive treatment for glioblastoma with photodynamic therapy
Project/Area Number |
25861278
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Neurosurgery
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Research Institution | Yamaguchi University |
Principal Investigator |
SADAHIRO Hirokazu 山口大学, 医学(系)研究科(研究院), 助教 (50509320)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Project Status |
Completed (Fiscal Year 2015)
|
Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
|
Keywords | 膠芽腫 / 5-ALA / PDD / PDT / 悪性脳腫瘍 / brain tumor stem cell / photodynamic diagnosis / photodynmic therapy / 5-aminolevulinic acid |
Outline of Final Research Achievements |
5-aminolevulinic acid (5-ALA) induced photodynamic diagnosis (PDD) and photodynamic therapy (PDT) are used for patients with glioblastoma multiforme (GBM). The goal of this study was to investigate the effects of 5-ALA-induced PDD and PDT in GS cells in vitro.Three GS cell lines (G144, G179 and Y10) were obtained as primary cultures from GBM specimens. Four hours after addition of 5-ALA, PDD was evaluated based on detection of PpIX fluorescence using flow cytometry. The effect of PDT on the cells was assessed after irradiation at 20 J/cm2 using an argon-pumped dye laser.PpIX was observed in more than 70% of GS cells from just after washing out of 5-ALA until 6 hours later. The GS cell death rate was 89% after PDT.5-ALA-induced PDD and PDT are very effective for GS cells in vitro. These photodynamic techniques may be useful for diagnosis and therapy associated with GS cells.
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Report
(4 results)
Research Products
(1 results)
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[Journal Article] Pathological features of highly invasive glioma stem cells in a mouse xenograft model.2013
Author(s)
Sadahiro, H., Yoshikawa, K., Ideguchi, M., Kajiwara, K., Ishii, A., Ikeda, E., Owada, Y., Yasumoto, Y. and Suzuki, M.
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Journal Title
Brain Tumor Pathol
Volume: 31
Issue: 2
Pages: 77-84
DOI
Related Report
Peer Reviewed