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New development of a functional analysis and the differentiation instruction therapy of the glioblastoma stem cells using in vivo light imaging

Research Project

Project/Area Number 25861280
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurosurgery
Research InstitutionEhime University

Principal Investigator

Inoue Akihiro  愛媛大学, 医学(系)研究科(研究院), 助教 (20593403)

Project Period (FY) 2013-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2015: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords脳腫瘍学 / Oct-3/4 / cancer stem cell / 血管新生阻害剤 / in vivo 光イメージング / HIF-1α / VEGF / 腫瘍幹細胞 / 血管新生
Outline of Final Research Achievements

In this study, we subcutaneously transplanted Oct-3/4-overexpressing human glioblastoma (U251/EGFP-Oct-3/4) cells into the right thighs of nude mice to evaluate the roles of Oct-3/4 in tumor angiogenesis. The conditioned media from U251/EGFP-Oct-3/4 cells significantly accelerated capillary-like tube formation compared with that of U251/EGFP cells. In comparison with U251/EGFP cells, U251/EGFP-Oct-3/4 cells had markedly elevated the expression of vascular endothelial growth factor mRNA under the control of hypoxia-inducible factor (HIF) 1α. In U251/EGFP-Oct-3/4 cells, enhanced protein expression and nuclear translocation of HIF1α were observed. Furthermore, we demonstrated that the involvement of AKT, an oncogenic signaling molecule, in the Oct-3/4 induced upregulation of HIF1α protein. Our findings suggest that Oct-3/4-expressing glioblastoma cells have the ability to adapt to low-oxygen environments within tumor masses by promoting tumor angiogenesis through AKT-HIF1 pathway.

Report

(4 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Research-status Report
  • 2013 Research-status Report
  • Research Products

    (10 results)

All 2015 2014 2013

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (8 results)

  • [Journal Article] Oct-3/4 promotes tumor angiogenesis through VEGF production in glioblastoma2014

    • Author(s)
      Takahashi H, Inoue A, Kawabe Y, et al.
    • Journal Title

      Brain Tumor Pathology

      Volume: 32 Issue: 1 Pages: 31-40

    • DOI

      10.1007/s10014-014-0203-3

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oct-3/4 modulates the drug-resistant phenotype of glioblastoma cells through expression of ATP binding cassette transporter G22014

    • Author(s)
      Hosokawa Y, Takahashi H, Inoue A, et al.
    • Journal Title

      Biochimica et Biophysica Acta

      Volume: 1850 Issue: 6 Pages: 1197-1205

    • DOI

      10.1016/j.bbagen.2015.01.017

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] 膠芽腫におけるOct-3/4とABC transporterの関連性2015

    • Author(s)
      井上 明宏、高野昌平、大上史朗、大西丘倫、高橋寿明、田中潤也
    • Organizer
      社団法人日本脳神経外科学会 第74回学術総会
    • Place of Presentation
      ロイトン札幌 北海道札幌市
    • Year and Date
      2015-10-14
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膠芽腫におけるOct-3/4のABC transporterを介した薬剤耐性機序への関与2015

    • Author(s)
      井上 明宏、高野昌平、大上史朗、大西丘倫、高橋寿明、田中潤也
    • Organizer
      第33回日本脳腫瘍病理学会
    • Place of Presentation
      JRホテルクレメント高松 香川県高松市
    • Year and Date
      2015-05-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 膠芽腫におけるOct-3/4とHIF-1の関連性2014

    • Author(s)
      井上明宏
    • Organizer
      第32回日本脳腫瘍学会
    • Place of Presentation
      千葉 シェラトン・グランデ・トーキョーベイ・ホテル
    • Year and Date
      2014-11-30 – 2014-12-02
    • Related Report
      2014 Research-status Report
  • [Presentation] 膠芽腫の血管新生におけるOct-3/4とHIF-1の関連性2014

    • Author(s)
      井上明宏
    • Organizer
      社団法人日本脳神経外科学会 第73回学術総会
    • Place of Presentation
      東京 グランドプリンスホテル新高輪
    • Year and Date
      2014-10-09 – 2014-10-11
    • Related Report
      2014 Research-status Report
  • [Presentation] 膠芽腫におけるOct-3/4を介した腫瘍増大機序について -HIF-1との関連性-2014

    • Author(s)
      井上明宏
    • Organizer
      第32回日本脳腫瘍病理学会
    • Place of Presentation
      徳島 あわぎんホール(徳島県郷土文化会館)
    • Year and Date
      2014-05-23 – 2014-05-24
    • Related Report
      2014 Research-status Report
  • [Presentation] 膠芽腫におけるOct-3/4のMGMT発現誘導機序への関与2013

    • Author(s)
      井上 明宏、その他
    • Organizer
      第31回日本脳腫瘍病理学会
    • Place of Presentation
      東京KFC Hall 国際ファッションセンター
    • Related Report
      2013 Research-status Report
  • [Presentation] Oct-3/4と膠芽腫に対するtemozolomide治療効果との関連性2013

    • Author(s)
      井上 明宏、その他
    • Organizer
      社団法人日本脳神経外科学会 第72回学術総会
    • Place of Presentation
      横浜 パシフィコ横浜
    • Related Report
      2013 Research-status Report
  • [Presentation] Oct-3/4のMGMT発現調節機構への関与2013

    • Author(s)
      井上 明宏、その他
    • Organizer
      第31回 日本脳腫瘍学会学術総会
    • Place of Presentation
      宮崎 フェニックス・シーガイア・リゾート
    • Related Report
      2013 Research-status Report

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Published: 2014-07-25   Modified: 2019-07-29  

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