| Project/Area Number |
25861284
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Neurosurgery
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUKUDA Shuji 長崎大学, 病院(医学系), 医員 (40646577)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | BBB / hyperglycemia / candesartan / pitavastatin / 高血糖 / GLP-1アナログ / タイトジャンクション蛋白 / シロスタゾール / ピタバスタチン |
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| Outline of Final Research Achievements |
Blood-brain barrier disruption represented a key feature in hyperglycemia (HG) -induced cerebral damage. The neuroprotective effect of cilostazol, pitavastatin, candesartan, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) has been demonstrated in animal models and clinical studies．However, it remained unclear whether they were effective for HG-induced BBB damage. We investigated the effect of cilostazol，pitavastatin，candesartan, EPA and DHA on HG-induced BBB damage. We used in vitro BBB models with primarily cultured of rat brain capillary endothelial cells (RBEC), and subjected cells to HG (55mM D-glucose). There was a significant decrease in transendothelial electrical resistance (TEER) under HG. There was not a significant decrease in cell viability under HG. Pitavastatin and cndesartan inhibited decreases in TEER induced by HG. HG compromised the barrier integrity of the in vitro BBB model. Pitavastatin and Candesartan improved HG-induced BBB damage．
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