| Project/Area Number |
25861304
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
KOZAWA Eiji 名古屋大学, 医学部附属病院, 病院助教 (60635572)
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| Research Collaborator |
NISHIDA Yoshihiro 名古屋大学, 医学系研究科, 准教授 (50332698)
URAKAWA Hiroshi 名古屋大学, 医学系研究科, 寄附講座講師 (60584753)
IKUTA Kunihiro 名古屋大学, 医学部附属病院, 医員 (40732657)
HAMADA Shunsuke 名古屋大学, 医学部附属病院, 医員 (90747289)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 腫瘍 / カテプシンK / 癌骨転移 / 遠隔転移 / 細胞・組織 / 軟骨・滑膜細胞 |
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| Outline of Final Research Achievements |
CatK synthesized by osteoclasts and/or tumor cells, tumor microenvironmental macrophage/ fibroblasts to be an obvious candidate for a key enzyme involved in the destruction of bone matrix.
To establish a murine bone metastasis model, a needle with syringe was used to inject MDA-MB-231 cells and Lewis lung cancer cells into the tibia of mice. Osteolytic lesions progressed markedly at tibia of mice. Scion image analysis revealed that the size of the osteolytic increased from 2 to 4 weeks. The expansion of bone lytic areas of tibia with Lewis lung cancer progressed rapidly for 2 weeks. Histological stain for bone tumor was performed.
To investigate the expression of Cathepsins, histological stain was performed on human cartilage and synovium. Increased expression of CatK in human OA cartilages/chondrocytes an synovial cells, compared with control cartilages/chondrocytes and synovial cells. Moreover, enzymatically activated CatK was also upregulated in OA chondrocytes.
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