| Project/Area Number |
25861309
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | CMC-PE / ＴＮＦ－α / nerve / pain / TNFα |
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| Outline of Final Research Achievements |
The purpose of this study is to develop a novel drug delivery system for nerve repair. We have developed a novel sodium carboxymethylcellulose (CMC)-derived hydrogel in which phosphatidylethanolamine (PE) was introduced into the carboxyl groups of CMC. We used a rat model of chronic nerve compression with silicon tube in sciatic nerve. Biomechanical analysis to assess perineural adhesion, electrophysiological evaluation of motor nerve conduction velocity (MCV), wet muscle weight measurements (tibialis anterior), and histological evaluation, morphometric analysis were performed at 1, 2 and 3 months after removal of silicone tube. These results suggest that the gel enhanced axonal recovery after chronic nerve compression. The result of real time PCR showed that the gel reduced expression of inflammatory cytokines. The gel may become the useful material for the drug delivery system.
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