The pathogenesis of steroid induced osteonecrosis at early onset using an osteonecrosis model rat

• Project/Area Number: 25861327
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: The University of Tokushima
• Principal Investigator: GOTO Tomohiro 徳島大学, ヘルスバイオサイエンス研究部, 特任講師 (10420548)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 大腿骨頭壊死 / ステロイド / in vivoバイオイメージング

Outline of Final Research Achievements

The presence of osteonecrosis was confirmed by detecting empty lacunae in histology of some specimens seven days after the last steroid administration. However, the occurrence of osteonecrosis in animal models was not constant. The positive HIF-1a was partially seen in the boundary region of the peripheral necrotic area. In MRI analysis, we have obtained enough resolution to analyze the occurrence of osteonecrosis in rat femur. However, alternative time differences between the occurrence of necrosis and detecting these lesions in MRI make difficult to do further analysis. In bio-imaging using Hypoxia probes, the positive findings in the steroid osteonecrosis rat model could not be obtained. Since it was observed variation in the creation of steroidal osteonecrosis rat model, confirmation of necrosis by image only appears to be incorrect. Thus currently, it is necessary to create more stable animal models in carrying forward this experiment.

Research Products

• [Journal Article] The state of the art in arthroscopic hip surgery (2014)
  • Author(s): Goto T, Hamada D, Mineta K, Tonogai I, Egawa H, Matsuura T, Takahashi M, Higashino K, Sakai T, Suzue N, Takata Y, Nishisho T, Goda Y, Sato R, Tezuka F, Kondo K, Takeuchi M, Sugiura K, Sairyo K
  • Journal Title: Journal of Medical Investigation Volume: 61 Pages: 226-232
  • NAID: 130004822726
  • Peer Reviewed / Open Access
• [Journal Article] Medial patellofemoral ligament reconstruction for patellar dislocation due to rupture of the medial structures after total knee arthroplasty: a case report and review of the literature (2014)
  • Author(s): Goto T, Hamada D, Iwame T, Suzue N, Takeuchi M, Egawa H, Sairyo K.
  • Journal Title: Journal of Medical Investigation Volume: 61 Pages: 409-412
  • NAID: 130004822750
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of hip arthroscopy in the diagnosis and treatment of synovial osteochondromatosis: a case report and literature review (2014)
  • Author(s): Mineta K, Goto T, Hamada D, Tsutsui T, Tonogai I, Suzue N, Matsuura T, Higashino K, Sakai T, Takata Y, Nishisho T, Sato R, Goda Y, Higuchi T, Hama S, Kimura T, Nitta A, Sairyo K.
  • Journal Title: Journal of Medical Investigation Volume: 61 Pages: 436-441
  • NAID: 130004822756
  • Peer Reviewed / Open Access
• [Journal Article] Bilateral atypical femoral fractures in a patient with multiple myeloma treated with intravenous bisphosphonate therapy (2014)
  • Author(s): Tonogai I, Goto T, Hamada D, Iwame T, Yoshioka S, Tsutsui T, Goda Y, Egawa H, Sairyo K.
  • Journal Title: Case reports in orthopedics Volume: 2014 Pages: 452418-452418
  • DOI: 10.1155/2014/452418
  • Peer Reviewed / Open Access
• [Journal Article] Massive femoral osteolysis secondary to loosening of a cemented roughened long stem: a case report (2014)
  • Author(s): Tamaki Y, Goto T, Hamada D, Nishisho T, Yukata K, Naoto S, Egawa H, Sairyo K.
  • Journal Title: Case reports in orthopedics Volume: 2014 Pages: 840267-840267
  • DOI: 10.1155/2014/840267
  • Peer Reviewed / Open Access
• [Presentation] 寛骨臼回転骨切り術前後における3次元的画像評価 (2014)
  • Author(s): 後東知宏，浜田大輔，筒井貴彦，峯田和明，西良浩一
  • Organizer: 第41回日本股関節学会
  • Place of Presentation: 京王プラザホテル（東京都）
  • Year and Date: 2014-10-31 – 2014-11-01
• [Presentation] Accuracy of CT based navigation system for cementless acetabular component placement in revision total hip arthroplasty (2014)
  • Author(s): Goto T, Mineta K, Hamada D, Sairyo K
  • Organizer: Asia pacific orthopaedic association
  • Place of Presentation: Pattaya, Thailand
  • Year and Date: 2014-10-23 – 2014-10-25
• [Presentation] CT画像を用いた股関節臼蓋縁骨化の評価 (2014)
  • Author(s): 後東知宏，峯田和明，浜田大輔，筒井貴彦，西良浩一
  • Organizer: 第6回日本関節鏡・膝・スポーツ整形外科学会
  • Place of Presentation: 広島国際会議場（広島県・広島市）
  • Year and Date: 2014-07-24 – 2014-07-26
• [Presentation] ＣＴを用いた大腿骨頚部嚢胞性病変の形態学的検討 (2014)
  • Author(s): 後東知宏，峯田和明，浜田大輔，江川洋史，西良浩一
  • Organizer: 第87回日本整形外科学会総会
  • Place of Presentation: 神戸国際会議場（兵庫県・神戸市）
  • Year and Date: 2014-05-22 – 2014-05-25
• [Presentation] Correlation between bone apposition of the acetabular rim and morphological indicators of femoroacetabular impingement: CT analysis in the Japanese asymptomatic population (2014)
  • Author(s): Goto T, Mineta K, Hamada D, Egawa H, Sairyo K
  • Organizer: OSTEOARTHRITIS RESEARCH SOCIETY INTERNATIONAL
  • Place of Presentation: Paris, France
  • Year and Date: 2014-04-24 – 2014-04-27