The functional analysis of perlecan in the late stage of osteophyte formation in knee osteoarthritis

• Project/Area Number: 25861343
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Orthopaedic surgery
• Research Institution: Juntendo University
• Principal Investigator: KANEKO Haruka 順天堂大学, 医学部, 助教 (50445516)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2013: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 変形性膝関節症 / 骨棘 / 滑膜 / 軟骨分化 / パールカン

Outline of Final Research Achievements

Osteophyte in knee osteoarthritis (knee OA) is formed from the mesenchymal cells present in the synovial membrane. The developmental process of osteophyte is similar to that of an endochondral ossification. However, it is still unclear their molecular mechanisms. Perlecan is a heparan sulfate proteoglycan present in the extracellular matrix of cartilage and synovium and is one of the essential factors for the cartilage development. The aim of this study was to investigate the role of perlecan expressed in the synovial membrane adjacent of the edge of articular cartilage in the late stage of osteophyte formation in knee OA. The absence of synovial perlecan suppressed the osteophyte formation in the late stage of knee OA. The results of the present study suggests that perlecan expressed in the synovial membrane promotes osteophyte formation in the late stage, in addition to the early stage, of knee OA.

Research Products

• [Presentation] パールカンはSOX9遺伝子発現制御を介して滑膜間葉系細胞の軟骨分化誘導を制御する (2015)
  • Author(s): 定月亮，石島旨章，金子晴香，羽田晋之介，木下真由子，金子和夫，平澤恵理
  • Organizer: 第47回日本結合組織学会学術大会
  • Place of Presentation: コクヨホール，東京
  • Year and Date: 2015-05-15 – 2015-05-16
• [Presentation] パールカンによる滑膜間葉系細胞の維持ならびに分化誘導の制御 (2014)
  • Author(s): 定月 亮, 石島 旨章, 金子 晴香, 二見 一平, 劉 立足, Yusup Anwarjan, 羽田 晋之介, 木下 真由子, Goldring Mary B., Yamada Yoshihiko, 平澤 恵理, 金子 和夫
  • Organizer: 第29回日本整形外科学会基礎学術集会
  • Place of Presentation: 城山観光ホテル，鹿児島
  • Year and Date: 2014-10-09 – 2014-10-10
• [Presentation] Role of perlecan in chondrogenic, osteogenic and adipogenic differentiation of synovial mesenchymal cells (2014)
  • Author(s): R. Sadatsuki, H. Kaneko, I. Futami, M. B. Goldring, Y. Yamada, E. Hirasawa, S. Hada, K. L. Culley, M. Otero, C. Dragomir, M. Kinoshita, L. Liu, A. Yusp, K. Kaneko, M. Ishijima
  • Organizer: 2014 World Congress on Osteoarthritis
  • Place of Presentation: Paris
• [Presentation] Perlecan is required for chondrogenic differentiation of mesenchymal synovial cells (2013)
  • Author(s): H. Kaneko, R. Sadatsuki, K. L. Culley, M. Otero, I. Futami, S. Hada, E. Hirasawa-Arikawa, Y. Yamada, K. Kaneko, M. B. Goldring, M. Ishijima
  • Organizer: 2013 World Congress on Osteoarthritis
  • Place of Presentation: Philadelphia