| Project/Area Number |
25861377
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Anesthesiology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KAWANO Hiroaki 徳島大学, ヘルスバイオサイエンス研究部, 徳島大学特別研究員 (90467811)
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| Project Period (FY) |
2013-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | イソフルラン / グルカゴン様ペプチドー１ / プレコンディショニング / 心筋保護作用 / 麻酔薬 / 虚血再潅流障害 / グルカゴン様ペプチド-1 / 虚血再灌流障害 |
|---|
| Outline of Final Research Achievements |
To assess the role of volatile anesthetic (isoflurane) and glucagon-like peptide-1 (GLP-1) in the protection from ischemia/reperfusion injury, we tested mice with isoflurane or exendine-4, GLP-1 agonist, and then exposed the mice to ischemia/reperfusion. Similar infarct size reductions were produced by treatment with isoflurane or exendine-4. The cardioprotective effects of these pharmacological preconditioning were abolished in GLP-1 antagonist pretreatment. Furthermore, apoptosis has been evaluated in these preconditionings and we found after 2 hours of reperfusion that apoptosis in myocardium at risk of ischemia is attenuated by preconditionings.
Additionally, to determine the downstream mediators of involved in isoflurane or GLP-1 preconditioning, we performed immunoblotting and realtime PCR using preconditioned samples.
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