Mechanisims of isoflurane-induced carrdiac protection from ischemia/reperfusion injury: a role for glucagon-like peptide-1.

• Project/Area Number: 25861377
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Anesthesiology
• Research Institution: The University of Tokushima
• Principal Investigator: KAWANO Hiroaki 徳島大学, ヘルスバイオサイエンス研究部, 徳島大学特別研究員 (90467811)
• Project Period (FY): 2013-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: イソフルラン / グルカゴン様ペプチドー１ / プレコンディショニング / 心筋保護作用 / 麻酔薬 / 虚血再潅流障害 / グルカゴン様ペプチド-1 / 虚血再灌流障害

Outline of Final Research Achievements

To assess the role of volatile anesthetic (isoflurane) and glucagon-like peptide-1 (GLP-1) in the protection from ischemia/reperfusion injury, we tested mice with isoflurane or exendine-4, GLP-1 agonist, and then exposed the mice to ischemia/reperfusion. Similar infarct size reductions were produced by treatment with isoflurane or exendine-4. The cardioprotective effects of these pharmacological preconditioning were abolished in GLP-1 antagonist pretreatment. Furthermore, apoptosis has been evaluated in these preconditionings and we found after 2 hours of reperfusion that apoptosis in myocardium at risk of ischemia is attenuated by preconditionings.
Additionally, to determine the downstream mediators of involved in isoflurane or GLP-1 preconditioning, we performed immunoblotting and realtime PCR using preconditioned samples.

Research Products

• [Journal Article] Impact of newly developed, next-generation artificial endocrine pancreas (2015)
  • Author(s): Noriko Kambe, Shinji Kawahito, Naoji Mita, Kazumi Takaishi, Toshiko Katayama, Yoko Sakai, Tomohiro Soga, Hiroaki Kawano、Munehide Matsuhisa, Mitsuo Shimada, Tetsuya Kitagawa, and Hiroshi Kitahata
  • Journal Title: The Journal of Medical Investigation Volume: 62 Issue: 1.2 Pages: 41-44
  • DOI: 10.2152/jmi.62.41
  • NAID: 130005129688
  • ISSN: 1343-1420, 1349-6867
  • Peer Reviewed / Open Access
• [Journal Article] Effects of a novel method of anesthesia combining propofol and volatile anesthesia on the incidence ofpostoperative nausea and vomiting in patientsundergoing laparoscopic gynecological surgery (2015)
  • Author(s): Hiroaki Kawano, Naohiro Ohshita, Kimiko Katome, Takako Kadota, Michiko Kinoshita, Yayoi Matsuoka, Yasuo M. Tsutsumi, Shinji Kawahito, Katsuya Tanaka, Shuzo Oshita
  • Journal Title: Revista Brasileira de Anesthesiologia Volume: 印刷中 Issue: 1 Pages: 12-18
  • DOI: 10.1016/j.bjane.2014.07.005
  • Peer Reviewed / Open Access
• [Journal Article] Anesthesia for arthroscopic shoulder surgery in the beach chair position: monitoring of cerebral oxygenation using combined bispectral index and near-infrared spectroscopy. (2014)
  • Author(s): Hiroaki Kawano, Tomomi Matsumoto
  • Journal Title: Middle East of Journal of Anaesthesiology Volume: 22 Pages: 613-617
  • Peer Reviewed