| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Neuroinflammation mediated by activated macrophages is important for pathogenesis of neuropathic pain. Here, we investigated the role of MMP12 in neuropathic pain. The gene expression of MMP12 was increased in the injured sciatic nerve (SCN), and MMP12 protein was localized on infiltrating macrophages in the injured site of SCN. By DiI labeling for peripheral blood vessels, DiI leaked into the surrounding tissue after injury, and macrophages accumulated around DiI labeled peripheral vessels. The expression of MMP12 was increased in LPS-activated macrophages. Perineural injection of macrophages depletion agent attenuated nerve injury-induced both tactile allodynia and thermal hyperalgesia, and suppressed the mRNA expressions of MMP12 and inflammatory cytokines. Furthermore, perineural injection of MMP12 specific inhibitor also suppressed tactile allodynia and thermal hyperalgesia. Taken together, MMP12 derived from macrophages acts as an exacerbating factor of neuropathic pain.
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